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首页> 外文期刊>Epigenetics: official journal of the DNA Methylation Society >VIM proteins regulate transcription exclusively through the MET1 cytosine methylation pathway
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VIM proteins regulate transcription exclusively through the MET1 cytosine methylation pathway

机译：VIM蛋白仅通过MET1胞嘧啶甲基化途径调节转录

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摘要

In Arabidopsis, variant in methylation (VIM) proteins are required for the maintenance of DNA methylation in the CpG dinucleotide context. VIM1 acts as a cofactor of DNA methyltransferase 1 (MET1), although the mechanism for this co-regulation remains unclear. In this study, we used RNA-seq analysis to profile the transcriptomes of vim1, vim1 vim2 vim3, and met1 null mutants. Consistent with previous studies indicating functional redundancy between these VIM proteins, we found no transcripts that were significantly misregulated in vim1 mutants. However, we identified a large set of VIM protein regulatory targets through analysis of vim1 vim2 vim3 mutants, and we observed that this set is essentially identical to that regulated by MET1. Log 2 fold changes in gene expression relative to wild type are strongly correlated between vim1 vim2 vim3 and met1 mutants. While the largest subset of these transcripts is upregulated and enriched with transposable elements, we also found small subsets of downregulated genes in each mutant, which are enriched with protein-coding genes. Together, these results expand on previous studies that profiled cytosine methylation in the vim1 vim2 vim3 mutant, and show that VIM proteins function in transcriptional regulation via their roles in the MET1 DNA methylation pathway.
机译：在拟南芥中，需要甲基化变异蛋白（VIM）来维持CpG二核苷酸环境中的DNA甲基化。 VIM1充当DNA甲基转移酶1（MET1）的辅助因子，尽管这种协同调节的机制尚不清楚。在这项研究中，我们使用RNA序列分析分析了vim1，vim1，vim2，vim3和met1无效突变体的转录组。与先前的研究表明这些VIM蛋白之间存在功能冗余的研究一致，我们没有发现在vim1突变体中存在显着失调的转录本。但是，我们通过对vim1 vim2 vim3突变体的分析鉴定了一大套VIM蛋白调节靶标，并且我们观察到该设置与MET1调节的基本上相同。基因表达相对于野生型的Log 2倍变化与vim1 vim2 vim3和met1突变体之间密切相关。这些转录物的最大子集被上调并富含转座因子，但我们还在每个突变体中发现了下调基因的小子集，这些子集富含蛋白质编码基因。在一起，这些结果扩展了以前的研究，该研究在vim1 vim2 vim3突变体中描述了胞嘧啶甲基化，并表明VIM蛋白通过其在MET1 DNA甲基化途径中的作用在转录调控中起作用。

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