首页> 外文期刊>Biochimica et biophysica acta. Gene structure and expression >Identification of 10Sa RNA (tmRNA) homologues from the cyanobacterium Synechococcus sp. strain PCC6301 and related organisms
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Identification of 10Sa RNA (tmRNA) homologues from the cyanobacterium Synechococcus sp. strain PCC6301 and related organisms

机译:从蓝藻Syechococcus sp。鉴定10Sa RNA(tmRNA)同源物。 PCC6301菌株和相关生物

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摘要

We have isolated the 10Sa RNA (tmRNA) from the unicellular cyanobacterium Synechococcus sp. strain PCC6301. It comprises of 394 nucleotides (nt) and has 55% homology to Escherichia coli tmRNA. The cloning and sequencing of the corresponding gene have revealed that, like in many tRNA genes, the terminal CCA sequence reported in all the tmRNA species characterized so far is not encoded in the DNA. Hybridization analysis has shown that the tmRNA gene is present as a single copy. Fairly high levels of tmRNA accumulate throughout the cell cycle; however, a slight increase in its level is observed during late-log to stationary phase. This suggests that tmRNA is functional not only when cells divide actively but also when cell growth stops.
机译:我们已经从单细胞蓝藻Synechococcus sp。中分离了10Sa RNA(tmRNA)。菌株PCC6301。它包含394个核苷酸(nt),与大肠杆菌tmRNA具有55%的同源性。相应基因的克隆和测序表明,与许多tRNA基因一样,到目前为止,表征的所有tmRNA物种中报道的末端CCA序列均未在DNA中编码。杂交分析表明,tmRNA基因以单拷贝形式存在。在整个细胞周期中积累了相当高水平的tmRNA;但是,在对数后期到静止阶段,其水平略有增加。这表明tmRNA不仅在细胞活跃分裂时起作用,而且在细胞生长停止时也起作用。

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