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首页> 外文期刊>Environmental Science: Nano >Gene expression as an indicator of the molecular response and toxicity in the bacterium Shewanella oneidensis and the water flea Daphnia magna exposed to functionalized gold nanoparticles
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Gene expression as an indicator of the molecular response and toxicity in the bacterium Shewanella oneidensis and the water flea Daphnia magna exposed to functionalized gold nanoparticles

机译：基因表达可作为暴露于功能化金纳米颗粒中的印度希瓦氏菌和跳蚤水蚤的分子反应和毒性的指标

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Nanoparticle (NP) physiochemical properties have been shown to be important determinants of NP interactions with biological systems. Due to both nanomaterial diversity and environmental complexity, a mechanistic understanding of how physiochemical properties affect NP/organism interactions will greatly aid in the accurate assessment and prediction of current and emerging NP-induced environmental impacts. Herein, we investigated key biological apical endpoints, such as viability, growth, and reproduction and the expression of genes associated with related molecular pathways in response to exposure to gold nanoparticles (AuNPs) functionalized with either positively charged ligands, polyallyamine hydrochloride, or negatively charged ligands, mercaptopropionic acid, in two model organisms, the bacterium Shewanella oneidensis MR-1 and the water flea Daphnia magna. By linking changes in molecular pathways to apical endpoints, potential biomarkers for functionalized AuNP impacts were identified in both organisms. Specifically, act was identified as a potential biomarker in D. magna and 16S as a potential biomarker in S. oneidensis. We also revealed that changes in molecular pathways induced by ligand-NP combination were strongly dependent upon the type of ligand on the NP surface, and the effects from their respective ligands alone might predict these effects for the ligand-NP combination, but only in some cases. Lastly, we revealed that it is possible to identify similar pathways provoked upon NP exposure across organisms. This study shows that molecular pathways will help elucidate mechanisms for NP toxicity that are predictive of adverse environmental outcomes.
机译：纳米粒子（NP）的物理化学性质已被证明是NP与生物系统相互作用的重要决定因素。由于纳米材料的多样性和环境的复杂性，对理化特性如何影响NP /生物相互作用的机械理解将大大有助于准确评估和预测当前和新出现的NP引起的环境影响。在这里，我们调查了关键的生物顶端终点，例如生存力，生长和繁殖以及与相关分子途径相关的基因的表达，以响应暴露于被带正电的配体，聚烯丙胺盐酸盐或带负电的功能的金纳米颗粒（AuNPs）的反应两种模式生物中的配体，巯基丙酸，即希瓦氏菌oneidensis MR-1和水蚤蚤。通过将分子途径的变化与顶端端点联系起来，在两种生物体中都发现了功能化AuNP影响的潜在生物标记。具体而言，行为被确定为是巨大的D. magna中的潜在生物标志物，而16S被确定为S. oneidensis中的潜在生物标志物。我们还揭示了由配体-NP组合诱导的分子途径变化强烈依赖于NP表面上的配体类型，单独来自其各自配体的作用可能预示了对配体-NP组合的作用。案件。最后，我们发现有可能确定跨生物体的NP暴露引起的类似途径。这项研究表明，分子途径将有助于阐明预测不良环境后果的NP毒性机制。

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