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首页> 外文期刊>Biochimica et biophysica acta. Biomembranes >Influence of cholesterol on electroporation of bilayer lipid membranes: chronopotentiometric studies
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Influence of cholesterol on electroporation of bilayer lipid membranes: chronopotentiometric studies

机译：胆固醇对双层脂质膜电穿孔的影响：计时电位研究

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This paper presents the results of constant-current (chronopotentiometric) measurements of the egg yolk phosphatidylcholine (PC) bilayer membrane without and with cholesterol. The experiments were performed on planar bilayer lipid membrane (BLM) formed by the Mueller–Rudin method. It is demonstrated that the constant-intensity current flow through bilayer membranes generated fluctuating pores in their structure. The presence of cholesterol in the membrane caused an increase in the value of the breakdown potential. It is postulated that greater stability of the bilayer with cholesterol can result from an increased critical pore radius (at which the bilayer would undergo irreversible rupture). This confirms that cholesterol has a stabilizing effect on BLM. Besides, our results suggest that addition of cholesterol causes shift in the distribution of pore conductance towards a smaller value. It is suggested that this can be connected with the phenomenon of domain formation in the membranes containing high concentration of cholesterol. Moreover, it is shown that chronopotentiometry with programmable current intensity is a promising method for observation of the membrane recovery process.
机译：本文介绍了恒定电流（计时电位法）测量不带胆固醇和带胆固醇的蛋黄磷脂酰胆碱（PC）双层膜的结果。实验是通过Mueller-Rudin方法形成的平面双层脂质膜（BLM）进行的。结果表明，恒定强度的电流流过双层膜会在其结构中产生波动的孔。膜中胆固醇的存在导致击穿电位值增加。据推测，双层具有胆固醇的更大稳定性可以由增加的临界孔半径（双层将经历不可逆的破裂）而导致。这证实了胆固醇对BLM具有稳定作用。此外，我们的结果表明，胆固醇的添加会导致孔隙电导率分布向较小的值移动。有人认为这可能与胆固醇含量高的膜的结构域形成现象有关。此外，显示了具有可编程电流强度的计时电位计是用于观察膜恢复过程的有前途的方法。

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