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首页> 外文期刊>Enzyme and Microbial Technology >Hydrolysis of orange peel by a pectin lyase-overproducing hybrid obtained by protoplast fusion between mutant pectinolytic Aspergillus flavipes and Aspergillus niveus CH-Y-1043
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Hydrolysis of orange peel by a pectin lyase-overproducing hybrid obtained by protoplast fusion between mutant pectinolytic Aspergillus flavipes and Aspergillus niveus CH-Y-1043

机译：果胶裂解酶高产杂种水解橙皮的过程，该杂种是通过突变果胶分解的黄曲霉黄曲霉菌CH-Y-1043与原生质体融合获得的

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摘要

Pectin lyases cleave the internal glycosidic bonds of pectin by β-elimination, producing non-saturated galacturonic oligomers. Genetic improvement of pectin lyase-overproducing strains is still necessary to improve industrial processes based on this enzyme. In the present study hybrids were obtained by protoplast fusion between mutant pectinolytic Aspergillus flavipes and Aspergillus niveus CH-Y-1043 strains. Prototrophic segregants showed different isoenzymatic profiles and produced increased levels of pectin lyase in cultures containing lemon peel as a sole carbon source. Hybrid HZ showed an increase of 450% and 1300% in pectin lyase production compared with that of A. niveus CH-Y-1043 and A. flavipes, respectively. Pectin lyase produced by the hybrid HZ was partially purified and used for the hydrolysis of orange peel. Pectin lyase was able to hydrolyze 56% of orange peel biomass. However, addition of 2 RFU and 20 U of endo- and exo-polygalacturonase, respectively, induced the hydrolysis of 92% of orange peel solids. In conclusion HZ is a pectin lyase-overproducing hybrid with potential applications in the pectin industry.
机译：果胶裂合酶通过β-消除作用裂解果胶的内部糖苷键，从而产生不饱和的半乳糖醛酸低聚物。果胶裂解酶高产菌株的遗传改良对于改善基于该酶的工业过程仍然是必需的。在本研究中，杂合体是通过突变的溶菌性黄曲霉黄曲霉菌株和n。Asivegillus niveus CH-Y-1043菌株之间的原生质体融合获得的。在含有柠檬皮作为唯一碳源的培养物中，原养的分离剂显示出不同的同工酶谱，并产生增加水平的果胶裂解酶。杂种HZ分别比n。niveus CH-Y-1043和A. flavipes的果胶裂解酶产量增加了450％和1300％。由杂种HZ产生的果胶裂解酶被部分纯化，并用于橙皮的水解。果胶裂解酶能够水解56％的桔皮生物质。但是，分别加入2 RFU和20 U内切和外切聚半乳糖醛酸酶可诱导92％的橙皮固体水解。总之，HZ是果胶裂解酶高产杂种，在果胶工业中具有潜在的应用前景。

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