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首页> 外文期刊>Biochimica et Biophysica Acta. Gene Regulatory Mechanisms >Monodehydroascorbate reductase gene, regulated by the wheat PN-2013 miRNA, contributes to adult wheat plant resistance to stripe rust through ROS metabolism
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Monodehydroascorbate reductase gene, regulated by the wheat PN-2013 miRNA, contributes to adult wheat plant resistance to stripe rust through ROS metabolism

机译：单脱氢抗坏血酸还原酶基因受小麦PN-2013 miRNA调控，有助于成年小麦植物通过ROS代谢抵抗条锈病

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摘要

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive wheat diseases worldwide. Varieties with adult plant resistance (APR) maintain effective and durable disease resistance. APR to stripe rust in wheat cultivar XZ9104 (XZ) is associated with extensive hypersensitive cell death and production of reactive oxygen species in the host. MDHAR is an important gene in the AsA-GSH cycle, and it plays an important role in maintaining the reduced pool of AsA scavenging hydrogen peroxide. microRNAs (miRNAs) were shown to engage in post-transcriptional regulation by degrading target mRNAs or repressing gene translation in plants responding to abiotic/biotic stresses. Previously, two novel miRNAs (1136-P3 and PN-2013) were isolated in wheat and the target gene of them was determined using degradome sequencing technology. In this study, the target gene was isolated and characterized as TaMDHAR, a monodehydroascorbate reductase gene. We first demonstrated that the target gene could be cleaved by these two miRNAs in tobacco leaves experimentally. However, TaMDHAR was regulated by PN-2013, not 1136-P3, in wheat-Pst adult incompatible interaction according to the expression patterns. The TaMDHAR knockdown resulted in improved wheat resistance to Pst at the seedling stage, with no influence on 1136-P3 and PN-2013 expression. The TaMDHAR knockdown resulted in a much greater H2O2 accumulation and lower APX and CAT activities together with higher expression in several PR genes. We deduced that TaMDHAR could contribute to the APR of XZ through ROS metabolism as regulated by the AsA-GSH cycle.
机译：小麦条锈菌引起的小麦条锈病f。 sp。小麦（Pst）是世界上最具破坏性的小麦疾病之一。具有成年植物抗性（APR）的品种保持有效和持久的抗病性。小麦品种XZ9104（XZ）中的条带锈病APR与宿主中广泛的超敏细胞死亡和活性氧物质的产生有关。 MDHAR是AsA-GSH循环中的重要基因，并且在维持AsA清除过氧化氢的减少池中起重要作用。 microRNA（miRNA）被证明可通过降解目标mRNA或抑制植物对非生物/生物胁迫的基因翻译来参与转录后调控。以前，在小麦中分离了两个新颖的miRNA（1136-P3和PN-2013），并使用降解组测序技术确定了它们的靶基因。在这项研究中，目标基因被分离并表征为TaMDHAR，一种单脱氢抗坏血酸还原酶基因。我们首先证明了实验基因可以通过烟草叶片中的这两个miRNA裂解靶基因。然而，根据表达方式，TaMDHAR受小麦-Pst成虫不相容相互作用的PN-2013调控，而不是1136-P3调控。 TaMDHAR基因敲低可提高小麦苗期对Pst的抗性，而不会影响1136-P3和PN-2013的表达。 TaMDHAR基因敲低导致更多的H2O2积累和更低的APX和CAT活性，以及​​在几个PR基因中的更高表达。我们推论出TaMDHAR可以通过ROS代谢（由AsA-GSH周期调节）来促进XZ的APR。

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