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首页> 外文期刊>Environmental microbiology >A novel mitochondrial membrane protein, Ohmm, limits fungal oxidative stress resistance and virulence in the insect fungal pathogen Beauveria bassiana
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A novel mitochondrial membrane protein, Ohmm, limits fungal oxidative stress resistance and virulence in the insect fungal pathogen Beauveria bassiana

机译：一种新型的线粒体膜蛋白Ohmm限制了昆虫真菌病原体球孢白僵菌中的真菌氧化应激抗性和毒力

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摘要

The Hog1 mitogen-activated protein (MAP) kinase regulates environmental stress responses and virulence in the entomopathogenic fungus Beauveria bassiana. To further characterize this pathway, we constructed a subtraction library enriched for genes regulated by Hog1. One targeted gene, encoding a novel membrane protein, Ohmm (oxidative homeostasis membrane-protein-mitochondria), was uniquely identified as being downregulated in the Hog1 background during growth under non-stress and osmotic stress conditions, but upregulated under oxidative stress. Ohmm was an experimentally validated flavin-binding protein and targeted to the mitochondria. Deletion of Ohmm resulted in increased oxidative stress resistance, whereas overexpression caused an opposite phenotype. The Ohmm showed accumulation of reactive oxygen species with alterations in cell wall composition and compatible solute accumulation evident as compared with the wild type parent. Conidiation was reduced >80%; however, conidia produced by the Ohmm strain germinated significantly faster than wild type cells. Insect bioassays using the greater wax moth revealed increased virulence for the Ohmm strain in both topical and intrahemocoel injection assays, indicating a negative effect of the presence of Ohmm with respect to pathogenesis. As predicted from our characterization, deletion of Ohmm in a Hog1 background rescued its oxidative sensitivity phenotype, confirming that Ohmm acts downstream of the Hog1 MAP-kinase.
机译：Hog1丝裂原激活蛋白（MAP）激酶调节昆虫病原真菌球孢白僵菌中的环境应激反应和毒力。为了进一步表征该途径，我们构建了一个减法文库，该文库富含Hog1调控的基因。一个目标基因，编码一种新型的膜蛋白Ohmm（氧化稳态膜蛋白-线粒体），在非胁迫和渗透胁迫条件下的生长过程中，在Hog1背景中被独特地下调，而在氧化胁迫下被上调。欧姆是经过实验验证的黄素结合蛋白，并靶向线粒体。欧姆的缺失导致增加的抗氧化应激性，而过表达引起相反的表型。与野生型亲本相比，Ohmm表现出活性氧物种的积累，且细胞壁组成发生了变化，相容性溶质积累明显。分娩率降低> 80％;但是，由Ohmm菌株产生的分生孢子发芽的速度明显快于野生型细胞。使用较大蜡蛾的昆虫生物测定法显示，在局部和内部hemocoel注射测定法中，Ohmm菌株的毒力均增加，表明Ohmm的存在对发病机理具有负面影响。根据我们的特征预测，在Hog1背景中删除Ohmm可恢复其氧化敏感性表型，从而确认Ohmm在Hog1 MAP激酶的下游起作用。

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