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首页> 外文期刊>Environmental microbiology >Field-based and laboratory stable isotope probing surveys of the identities of both aerobic and anaerobic benzene-metabolizing microorganisms in freshwater sediment
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Field-based and laboratory stable isotope probing surveys of the identities of both aerobic and anaerobic benzene-metabolizing microorganisms in freshwater sediment

机译：野外和实验室稳定同位素探测调查淡水沉积物中需氧和厌氧的苯代谢微生物的身份

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Laboratory incubations of coal-tar waste-contaminated sediment microbial communities under relatively controlled physiological conditions were used to interpret results of a field-based stable isotope probing (SIP) assay. Biodegradation activity of C-13-benzene was examined by GC/MS determination of net (CO2)-C-13 production and by GC headspace analysis of benzene loss. Key experimental variables were: the site of the assays (laboratory serum-bottle incubations and in situ field sediments), benzene concentration (10, 36 or 200 p.p.m. in laboratory assays), and physiological conditions (anaerobic with or without sulfate or nitrate additions versus aerobic headspace or the uncontrolled field). In anaerobic laboratory incubations of benzene at 10 p.p.m., greater than 60% of the substrate was eliminated within 15 days. During anaerobic incubations of 200 p.p.m. benzene (70 days), 0.9% benzene mineralization occurred. When benzene (36 p.p.m.) was added to sediment with air in the serum-bottle headspace, 14% of the initial C-13 was mineralized to (CO2)-C-13 in 2.5 days. In the field experiment (178 mu g C-13-benzene dosed to undisturbed sediments), net (CO2)-C-13 production reached 0.3% within 8.5 h. After isopycnic separation of C-13 (heavy)-labelled DNA from the above biodegradation assays, sequencing of C-13-DNA clone libraries revealed a broad diversity of taxa involved in benzene metabolism and distinctive libraries for each biodegradation treatment. Perhaps most importantly, in the field SIP experiment the clone libraries produced were dominated by Pelomonas (betaproteobacteria) sequences similar to those found in the anaerobic 10 p.p.m. benzene laboratory experiment. These data indicate that the physiological conditions that prevail and govern in situ biodegradation of pollutants in the field may be interpreted by knowing the physiological preferences of potentially active populations.
机译：在相对受控的生理条件下，对煤焦油废物污染的沉积物微生物群落进行实验室培养，以解释基于现场的稳定同位素探测（SIP）分析的结果。通过GC / MS测定净（CO2）-C-13产生量和通过GC顶空分析苯损失来检查C-13-苯的生物降解活性。关键的实验变量是：测定部位（实验室血清瓶培养和原位田间沉积物），苯浓度（实验室测定中为10、36或200 ppm）和生理条件（厌氧，添加或不添加硫酸盐或硝酸盐与有氧的顶空或不受控制的场）。在10 p.p.m.的厌氧实验室孵育中，在15天内消除了60％以上的底物。在200 p.p.m.的厌氧培养中苯（70天），发生0.9％的苯矿化。当在血清瓶顶部空间中的空气中将苯（36 p.p.m.）添加到沉积物中时，在2.5天内将14％的初始C-13矿化为（CO2）-C-13。在田间试验中（向未扰动的沉积物中添加了178微克C-13-苯），在8.5小时内净（CO2）-C-13产量达到0.3％。从上述生物降解试验中等密度分离C-13（重）标记的DNA后，对C-13-DNA克隆文库的测序显示，参与苯代谢的分类单元种类繁多，每种生物降解处理均具有独特的文库。也许最重要的是，在现场SIP实验中，所产生的克隆文库被Pelomonas（β变形杆菌）序列所控制，该序列类似于在厌氧10 p.p.m中发现的序列。苯实验室实验。这些数据表明，可以通过了解潜在活跃人群的生理偏好来解释在田间普遍存在并控制污染物在现场进行生物降解的生理条件。

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