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首页> 外文期刊>Environmental microbiology >Shewanella oneidensis MR-1 mutants selected for their inability to produce soluble organic-Fe(III) complexes are unable to respire Fe(III) as anaerobic electron acceptor
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Shewanella oneidensis MR-1 mutants selected for their inability to produce soluble organic-Fe(III) complexes are unable to respire Fe(III) as anaerobic electron acceptor

机译：由于无法产生可溶性有机-Fe（III）配合物而被选择的Shewanella oneidensis MR-1突变体无法将Fe（III）作为厌氧电子受体

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Recent voltammetric analyses indicate that Shewanella putrefaciens strain 200 produces soluble organic-Fe(III) complexes during anaerobic respiration of sparingly soluble Fe(III) oxides. Results of the present study expand the range of Shewanella species capable of producing soluble organic-Fe(III) complexes to include Shewanella oneidensis MR-1. Soluble organic-Fe(III) was produced by S. oneidensis cultures incubated anaerobically with Fe(III) oxides, or with Fe(III) oxides and the alternate electron acceptor fumarate, but not in the presence of O-2, nitrate or trimethylamine-N-oxide. Chemical mutagenesis procedures were combined with a novel Micro Electrode Screening Array (MESA) to identify four (designated Sol) mutants with impaired ability to produce soluble organic-Fe(III) during anaerobic respiration of Fe(III) oxides. Two of the Sol mutants were deficient in anaerobic growth on both soluble Fe(III)-citrate and Fe(III) oxide, yet retained the ability to grow on a suite of seven alternate electron acceptors. The rates of soluble organic-Fe(III) production were proportional to the rates of iron reduction by the S. oneidensis wild-type and Sol mutant strains, and all four Sol mutants retained wild-type siderophore production capability. Results of this study indicate that the production of soluble organic-Fe(III) may be an important intermediate step in the anaerobic respiration of both soluble and sparingly soluble forms of Fe(III) by S. oneidensis.
机译：最近的伏安分析表明，腐臭希瓦氏菌200菌株在难溶性难溶性Fe（III）氧化物的厌氧呼吸过程中会产生可溶性有机-Fe（III）配合物。本研究的结果扩大了能够产生可溶性有机-Fe（III）配合物的希瓦氏菌物种的范围，使其包括雪希氏菌MR-1。可溶性有机铁（Fe）是由沙门氏菌培养物与Fe（III）氧化物，Fe（III）氧化物和替代电子受体富马酸盐厌氧孵育而产生的，但在O-2，硝酸盐或三甲胺的存在下不产生-N-氧化物化学诱变程序与新型微电极筛选阵列（MESA）相结合，以鉴定四个（指定的Sol）突变体，这些突变体在氧化Fe（III）的厌氧呼吸过程中产生可溶性有机Fe（III）的能力受损。两个Sol突变体在可溶性Fe（III）柠檬酸盐和Fe（III）氧化物上均缺乏厌氧生长能力，但仍保留了在一组七个替代电子受体上生长的能力。可溶性有机铁（III）的产生速率与沙门氏菌野生型和Sol突变菌株对铁的还原速率成正比，并且所有四个Sol突变体均保留了野生型铁载体的生产能力。这项研究的结果表明，可溶性有机-Fe（III）的产生可能是拟南芥对厌氧呼吸可溶性和少量可溶性形式的Fe（III）的重要中间步骤。

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