• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental microbiology >TRI6 and TRI10 play different roles in the regulation of deoxynivalenol (DON) production by cAMP signalling in Fusarium graminearum
【24h】

TRI6 and TRI10 play different roles in the regulation of deoxynivalenol (DON) production by cAMP signalling in Fusarium graminearum

机译：TRI6和TRI10在禾谷镰刀菌中通过cAMP信号调节脱氧雪腐烯（DON）产生的调节作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The biosynthesis of mycotoxin deoxynivalenol (DON) in Fusarium graminearum is regulated by two pathway-specific transcription factors Tri6 and Tri10 and affected by various host and environmental factors. In this study, we showed that cyclic adenosine monophosphate (cAMP) treatment induced DON production by stimulating TRI gene expression and DON-associated cellular differentiation in F. graminearum. Interestingly, exogenous cAMP had no effects on the tri6 mutant but partially recovered the defect of tri10 mutant in DON biosynthesis. Although the two cAMP phosphodiesterase genes PDE1 and PDE2 had overlapping functions in vegetative growth, conidiation, sexual reproduction and plant infection, deletion of PDE2 but not PDE1 activated intracellular PKA activities and increased DON production. Whereas the tri6 pde2 mutant failed to produce DON, the tri10 pde2 double mutant produced a significantly higher level of DON than the tri10 mutant. Cellular differentiation associated with DON production was stimulated by exogenous cAMP or deletion of PDE2 in both tri10 and tri6 mutants. These data indicate that TRI6 is essential for the regulation of DON biosynthesis by cAMP signalling but elevated PKA activities could partially bypass the requirement of TRI10 for TRI gene-expression and DON production, and Pde2 is the major cAMP phosphodiesterase to negatively regulate DON biosynthesis in F. graminearum.
机译：禾谷镰刀菌中霉菌毒素脱氧雪腐烯醇（DON）的生物合成受到两个途径特异性转录因子Tri6和Tri10的调控，并受到各种宿主和环境因素的影响。在这项研究中，我们表明，环磷酸一磷酸腺苷（cAMP）处理可通过刺激禾谷镰刀菌TRI基因表达和与DON相关的细胞分化来诱导DON的产生。有趣的是，外源性cAMP对tri6突变体没有影响，但部分弥补了DON生物合成中tri10突变体的缺陷。尽管两个cAMP磷酸二酯酶基因PDE1和PDE2在营养生长，分生，有性繁殖和植物感染中具有重叠的功能，但PDE2的缺失而不激活PDE1激活了细胞内PKA活性并增加了DON的产生。 tri6 pde2突变体无法产生DON，而tri10 pde2双突变体产生的DON水平却高于tri10突变体。在tri10和tri6突变体中，外源性cAMP或PDE2的缺失会刺激与DON产生相关的细胞分化。这些数据表明，TRI6对于通过cAMP信号调节DON生物合成至关重要，但升高的PKA活性可以部分绕过TRI10对TRI基因表达和DON产生的需求，而Pde2是主要的cAMP磷酸二酯酶，负调控F中的DON生物合成。禾本科

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号