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首页> 外文期刊>European journal of neurology: the official journal of the European Federation of Neurological Societies >Antibodies to heteromeric glycolipid complexes in multifocal motor neuropathy
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Antibodies to heteromeric glycolipid complexes in multifocal motor neuropathy

机译：多灶性运动神经病中异聚糖脂复合物的抗体

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Background: Measurement of anti-GM1 IgM antibodies in multifocal motor neuropathy (MMN) sera is confounded by relatively low sensitivity that limits clinical usefulness. Combinatorial assay methods, in which antibodies react to heteromeric complexes of two or more glycolipids, are being increasingly applied to this area of diagnostic testing. Methods: A newly developed combinatorial glycoarray able to identify antibodies to 45 different heteromeric glycolipid complexes and their 10 individual glycolipid components was applied to a randomly selected population of 33 MMN cases and 57 normal or disease controls. Comparison with an enzyme-linked immunosorbent assay (ELISA) was conducted for selected single glycolipids and their complexes. Results: By ELISA, 22/33 MMN cases had detectable anti-GM1 IgM antibodies, whereas 19/33 MMN samples were positive for anti-GM1 antibodies by glycoarray. Analysis of variance (anova) revealed that of the 55 possible single glycolipids and their 1:1 complexes, antibodies to the GM1:galactocerebroside (GM1:GalC) complex were most significantly associated with MMN, returning 33/33 MMN samples as positive by glycoarray and 29/33 positive by ELISA. Regression analysis revealed a high correlation in absolute values between ELISA and glycoarray. Receiver operator characteristic analysis revealed insignificantly different diagnostic performance between the two methods. However, the glycoarray appeared to offer slightly improved sensitivity by identifying antibodies in four ELISA-negative samples. Conclusions: The use of combinatorial glycoarray or ELISA increased the diagnostic sensitivity of anti-glycolipid antibody testing in this cohort of MMN cases, without significantly affecting specificity, and may be a useful assay modification for routine clinical screening.
机译：背景：多灶性运动神经病（MMN）血清中抗GM1 IgM抗体的测量因灵敏度相对较低而受到限制，从而限制了临床实用性。抗体与两种或多种糖脂异源复合物反应的组合检测方法正越来越多地应用于诊断测试领域。方法：将能够识别针对45种不同异聚糖脂复合物及其10种单独糖脂成分的抗体的新开发的组合糖阵列应用于随机选择的33例MMN病例和57例正常或疾病对照人群。与酶联免疫吸附测定法（ELISA）进行了比较，以选择单个糖脂及其复合物。结果：通过ELISA，22/33 MMN病例具有可检测的抗GM1 IgM抗体，而19/33 MMN样品经糖阵列检测为抗GM1抗体阳性。方差分析（方差分析）显示，在55种可能的单一糖脂及其1：1复合物中，针对GM1：半乳糖脑苷（GM1：GalC）复合物的抗体与MMN的关联最为明显，糖阵列将33/33 MMN样品作为阳性ELISA检测结果为29/33阳性。回归分析显示ELISA和糖阵列之间的绝对值高度相关。接收者操作员特征分析显示两种方法之间的诊断性能差异不明显。但是，通过鉴定四个ELISA阴性样品中的抗体，糖阵列似乎提供了稍微改善的敏感性。结论：在这一MMN病例队列中，组合糖阵列或ELISA的使用增加了抗糖脂抗体检测的诊断敏感性，而没有显着影响特异性，并且可能是常规临床筛查的有用的测定方法。

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