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首页> 外文期刊>Bioelectromagnetics. >EGF receptor clustering is induced by a 0.4 mT power frequency magnetic field and blocked by the EGF receptor tyrosine kinase inhibitor PD153035.
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EGF receptor clustering is induced by a 0.4 mT power frequency magnetic field and blocked by the EGF receptor tyrosine kinase inhibitor PD153035.

机译：EGF受体聚集是由0.4 mT工频磁场引起的，并被EGF受体酪氨酸激酶抑制剂PD153035阻断。

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摘要

Atomic force microscopy (AFM), transmission electron microscopy (TEM), and confocal laser scanning microscopy were used to investigate the effects of a 50 Hz 0.4 mT magnetic field (MF) on the clustering of purified epidermal growth factor receptors (EGFRs) and EGFRs in Chinese hamster lung (CHL) cell membrane. The results demonstrate that exposing purified EGFRs to the MF for 30 min induces receptor clustering. The peak height of apparent clusters increased from 1.42 +/- 0.18 (sham-exposed) to 3.08 +/- 0.38 nm (exposed) while the mean half-width increased from 21.7 +/- 2.2 to 33.0 +/- 4.0 nm. A similar effect was also observed by TEM. Treatment of purified EGFR with PD153035 (PD), an EGFR-specific tyrosine kinase (TK) inhibitor, inhibited the MF-induced EGFR clustering of the purified proteins, an effect also observed for the receptors in cell membrane in the absence of EGF. These results strongly suggest that the 50 Hz 0.4 mT MF interferes with the EGFR signaling pathway, most likely by interacting with the cytoplasmic TK domain.
机译：原子力显微镜（AFM），透射电子显微镜（TEM）和共聚焦激光扫描显微镜用于研究50 Hz 0.4 mT磁场（MF）对纯化的表皮生长因子受体（EGFRs）和EGFR聚集的影响在中国仓鼠肺（CHL）细胞膜中。结果表明，将纯化的EGFRs暴露于MF 30分钟会诱导受体聚集。表观簇的峰高从1.42 +/- 0.18 nm（假暴露）增加到3.08 +/- 0.38 nm（暴露），而平均半峰宽从21.7 +/- 2.2增加到33.0 +/- 4.0 nm。通过TEM也观察到类似的效果。用EGFR特异的酪氨酸激酶（TK）抑制剂PD153035（PD）处理纯化的EGFR，可抑制MF诱导的纯化蛋白的EGFR簇集，在没有EGF的情况下，也可观察到细胞膜受体的作用。这些结果强烈表明，50 Hz 0.4 mT MF最有可能通过与细胞质TK结构域相互作用而干扰EGFR信号通路。

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