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首页> 外文期刊>European journal of clinical microbiology and infectious diseases: Official publication of the European Society of Clinical Microbiology >Microbial identification and automated antibiotic susceptibility testing directly from positive blood cultures using MALDI-TOF MS and VITEK 2
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Microbial identification and automated antibiotic susceptibility testing directly from positive blood cultures using MALDI-TOF MS and VITEK 2

机译：使用MALDI-TOF MS和VITEK 2直接从阳性血液培养物中进行微生物鉴定和自动化抗生素敏感性测试

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The study addresses the utility of Matrix Assisted Laser Desorption/Ionisation Time-Of-Flight mass spectrometry (MALDI-TOF MS) using VITEK MS and the VITEK 2 antimicrobial susceptibility testing (AST) system for direct identification (ID) and timely AST from positive blood culture bottles using a lysis-filtration method (LFM). Between July and December 2014, a total of 140 non-duplicate mono-microbial blood cultures were processed. An aliquot of positive blood culture broth was incubated with lysis buffer before the bacteria were filtered and washed. Micro-organisms recovered from the filter were first identified using VITEK MS and its suspension was used for direct AST by VITEK 2 once the ID was known. Direct ID and AST results were compared with classical methods using solid growth. Out of the 140 bottles tested, VITEK MS resulted in 70.7 % correct identification to the genus and/or species level. For the 103 bottles where identification was possible, there was agreement in 97 samples (94.17 %) with classical culture. Compared to the routine method, the direct AST resulted in category agreement in 860 (96.5 %) of 891 bacteria-antimicrobial agent combinations tested. The results of direct ID and AST were available 16.1 hours before those of the standard approach on average. The combined use of VITEK MS and VITEK 2 directly on samples from positive blood culture bottles using a LFM technique can result in rapid and reliable ID and AST results in blood stream infections to result in early institution of targeted treatment. The combination of LFM and AST using VITEK 2 was found to expedite AST more reliably.
机译：该研究使用VITEK MS和VITEK 2抗菌药敏测试（AST）系统直接识别（ID）并及时从阳性结果进行AST来解决基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）的实用性血液培养瓶采用裂解过滤法（LFM）。在2014年7月至2014年12月之间，共处理了140种非重复的单微生物血液培养物。将阳性血液培养液的等分试样与裂解缓冲液温育，然后过滤并洗涤细菌。首先使用VITEK MS鉴定从过滤器中回收的微生物，一旦知道ID，其悬浮液便被VITEK 2用于直接AST。直接ID和AST结果与使用固体生长的经典方法进行了比较。在测试的140个瓶子中，VITEK MS对属和/或物种水平的正确识别率为70.7％。对于可以识别的103瓶，有97个样品（94.17％）与古典文化相符。与常规方法相比，直接AST在测试的891种细菌-抗菌剂组合中，有860种（96.5％）达到了类别一致性。直接ID和AST的结果平均比标准方法的结果晚了16.1小时。使用LFM技术将VITEK MS和VITEK 2直接结合在阳性血液培养瓶的样品上，可以快速，可靠地获得ID，而AST则导致血液感染，从而可以及早实施靶向治疗。发现使用VITEK 2的LFM和AST的组合可以更可靠地加速AST。

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