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首页> 外文期刊>European journal of gynaecological oncology >Inhibitory effects of c-erbB-2 antisense oligonucleotide transfection on uterine endometrial cancer Ishikawa cell lines.
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Inhibitory effects of c-erbB-2 antisense oligonucleotide transfection on uterine endometrial cancer Ishikawa cell lines.

机译：c-erbB-2反义寡核苷酸转染对子宫内膜癌石川细胞株的抑制作用。

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BACKGROUND: C-erbB-2 is a chief proto-oncogene of endometrial cancer, which plays an important role in the biological behavior of endometrial cancer. Its overespression is an important factor of poor progrosis. The objective of this study was to investigate the treatment effects of transfecting c-erbB-2 antisense oligonucleotide (ASODN) on the uterine endometrial cancer Ishikawa cell line. MATERIALS AND METHODS: The c-erbB-2 expression on Ishikawa cell membranes was determined by immunohistochemistry and then the aim was to transfect ASODN into Ishikawa cells and assay the cellular growth inhibition by MTT, to observe the cellular ultrastructure changes under transmission electron microscope (TEM), and to assay the cellular apoptotic rate, c-erbB-2 mRNA and protein expression by flow cytometry, RT-PCR and Western blot, respectively. RESULTS: C-erbB-2 protein was positively expressed on Ishikawa cell membranes. MTT showed that when the concentration of transfecting ASODNs was 0.3 uM and 0.6 uM, cell growth inhibition rates were 55.43% and 76.12%, respectively. After transfecting 0.3 uM ASODN, the Ishikawa cell ultrastructure was obviously damaged, the cellular apoptotic rate was 72.21%, and the c-erbB-2 mRNA and protein expression were 45.71% and 34.52%, respectively, compared with those of the normal control cells. CONCLUSIONS: Transfecting c-erbB-2 ASODN can obviously suppress its mRNA and protein expression in Ishikawa cells, cause cellular apoptosis and inhibit cell growth. It may have an important role in the gene therapy of endometrial cancer.
机译：背景：C-erbB-2是子宫内膜癌的主要原癌基因，在子宫内膜癌的生物学行为中起重要作用。它的过度表达是促生长不良的重要因素。这项研究的目的是研究转染c-erbB-2反义寡核苷酸（ASODN）对子宫内膜癌石川细胞株的治疗作用。材料与方法：采用免疫组织化学方法检测石川细胞膜上c-erbB-2的表达，目的是将ASODN转染石川细胞，MTT法检测其对细胞生长的抑制作用，在透射电镜下观察细胞超微结构的变化（ TEM），并分别通过流式细胞仪，RT-PCR和Western blot检测细胞凋亡率，c-erbB-2 mRNA和蛋白表达。结果：C-erbB-2蛋白在石川细胞膜上阳性表达。 MTT表明，当转染的ASODNs的浓度为0.3uM和0.6uM时，细胞生长抑制率分别为55.43％和76.12％。转染0.3 uM ASODN后，石川细胞的超微结构明显受损，细胞凋亡率为72.21％，c-erbB-2 mRNA和蛋白表达分别为正常对照组的45.71％和34.52％。 。结论：转染c-erbB-2 ASODN可明显抑制其在石川细胞中的mRNA和蛋白表达，引起细胞凋亡并抑制细胞生长。它可能在子宫内膜癌的基因治疗中起重要作用。

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