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首页> 外文期刊>European Journal of Histochemistry >Expression pattern of thymosin beta 4 in the adult human liver
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Expression pattern of thymosin beta 4 in the adult human liver

机译：胸腺素β4在成人肝脏中的表达模式

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摘要

Thymosin beta4 (Tβ4) is a member of beta-thymosins, a family of small peptides involved in polymerization of G-actin, and in many critical biological processes including apoptosis, cell migration, angiogenesis, and fibrosis. Previous studies in the newborn liver did not reveal any significant reactivity for Tβ4 during the intrauterine life. The aim of the present study was to investigate by immunohistochem-istry Tβ4 expression in the adult normal liver. Thirty-five human liver samples, including 11 needle liver biopsies and 24 liver specimens obtained at autopsy, in which no pathological change was detected at the histological examination, were immunostained utilizing an anti-Tβ4 commercial antibody. Tp4 was detected in the hepatocytes of all adult normal livers examined. A zonation of T(34 expression was evident in the vast majority of cases. Immunostaining was preferentially detected in zone 3, while a minor degree of reactivity was detected in periportal hepatocytes (zone 1). At higher power, Tβ4-reactive granules appeared mainly localized at the biliary pole of hepatocytes. In cases with a strong immunostaining, even perinuclear areas and the sinusoidal pole of hepatocytes appeared interested by immunoreactivity for Tβ4. The current work first evidences a strong diffuse expression of Tβ4 in the adult human liver, and adds hepatocytes to the list of human cells able to synthesize large amounts of Tβ4 in adulthood. Moreover, T(i4 should be added to the liver proteins characterized by a zonate expression pattern, in a descending gradient from the terminal vein to the periportal areas of the liver acinus. Identifying the intimate role played by this peptide intracellularly and extracellularly, in physiology and in different liver diseases, is a major challenge for future research focusing on Tβ4.
机译：胸腺素β4（Tβ4）是β-胸腺素的成员，β-胸腺素是参与G-肌动蛋白聚合以及许多关键生物学过程（包括细胞凋亡，细胞迁移，血管生成和纤维化）的小肽家族。先前在新生儿肝脏中进行的研究并未显示出子宫内生命期间对Tβ4的任何显着反应。本研究的目的是通过免疫组织化学Tβ4在成人正常肝脏中的表达进行研究。使用抗-Tβ4商业抗体对35例人类肝脏样品进行了免疫染色，其中包括11例穿刺肝活检和24例尸体解剖获得的肝标本，其中在组织学检查中未发现病理变化。在所有检查的成人正常肝脏的肝细胞中均检测到Tp4。在大多数情况下，T（34）表达明显分区。在3区优先检测免疫染色，而在门静脉周围的肝细胞（1区）中检测到较小的反应性。在较高功率下，Tβ4反应性颗粒主要出现免疫染色强烈的情况下，甚至对Tβ4的免疫反应性，即使是肝周区域和肝细胞的正弦极也表现出兴趣，目前的工作首先证明了Tβ4在成人肝脏中的强烈弥散表达，并增加了Tβ4的表达。肝细胞是成年后能够合成大量Tβ4的人类细胞列表。此外，应将T（i4）以特征性带状表达的形式添加到肝蛋白中，并从终末静脉到门静脉周围区域呈递减的梯度鉴定该肽在细胞内和细胞外，在生理学和不同肝病中的亲密作用是主要的着眼于Tβ4的未来研究。

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