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首页> 外文期刊>European Journal of Histochemistry >Immunohistochemical evidence: Testicular and scented glandular androgen synthesis in muskrats (Ondatra zibethicus) during the breeding season
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Immunohistochemical evidence: Testicular and scented glandular androgen synthesis in muskrats (Ondatra zibethicus) during the breeding season

机译：免疫组织化学证据：繁殖季节麝香（Ondatra zibethicus）睾丸和雄性腺雄激素的合成

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摘要

In order to elucidate the relationship between androgens and the function of the muskrat (Ondatra zibethicus) scented glands during the breeding season, we investigated immunolocalization of steroidogenic enzymes P450scc, 3βHSD and P450c17 in the muskrat testes and scented glands. Nine adult muskrats were obtained in March (n=3), May (n=3) and July (n=3) 2010. Steroidogenic enzymes were immunolocalized using polyclonal antisera raised against bovine adrenal P450scc, human placental 3βHSD and porcine testicular P450c17. Histologically, all types of spermatogenic cells including mature-phase spermatozoa in seminiferous tubules were observed in all testes. Glandular cells, interstitial cells, epithelial cells and excretory tubules were identified in scented glands during the breeding season. P450scc, 3βHSD and P450c17 were only identified in Leydig cells during the breeding season; P450scc and P450c17 were observed in glandular cells of scented glands, however, 3βHSD was not found in scented glands during the breeding season. These novel findings provide the first evidence showing that scented glands of the muskrats are capable of locally synthesizing androgens and androgens acting via an endocrine, autocrine or paracrine manner may play an important role in scented gland function during the breeding season.
机译：为了阐明在繁殖季节中雄激素与麝香（Ondatra zibethicus）香腺的功能之间的关系，我们调查了类固醇生成酶P450scc，3βHSD和P450c17在麝香鼠的睾丸和香腺中的免疫定位。在2010年3月（n = 3），5月（n = 3）和7月（n = 3）获得了9只成年麝香。使用针对牛肾上腺P450scc，人胎盘3βHSD和猪睾丸P450c17的多克隆抗血清对类固醇酶进行了免疫定位。组织学上，在所有睾丸中均观察到所有类型的生精细胞，包括生精小管中的成熟期精子。在繁殖季节，在有气味的腺体中发现了腺细胞，间质细胞，上皮细胞和小管。 P450scc，3βHSD和P450c17仅在繁殖季节的Leydig细胞中鉴定到。在有气味的腺的腺细胞中观察到P450scc和P450c17，但是在繁殖季节在有气味的腺中未发现3βHSD。这些新颖的发现提供了第一个证据，表明麝香的气味腺能够局部合成雄激素和通过内分泌，自分泌或旁分泌方式起作用的雄激素可能在繁殖季节的气味腺功能中发挥重要作用。

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