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首页> 外文期刊>European Journal of Histochemistry >Transfer of Fas (CD95) protein from the cell surface to the surface of polystyrene beads coated with anti-Fas antibody clone CH-11
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Transfer of Fas (CD95) protein from the cell surface to the surface of polystyrene beads coated with anti-Fas antibody clone CH-11

机译：Fas（CD95）蛋白从细胞表面转移到涂有抗Fas抗体克隆CH-11的聚苯乙烯珠表面

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摘要

Mouse monoclonal anti-Fas (CD95) antibody clone CH-11 has been widely used in research on apoptosis. CH-11 has the ability to bind to Fas protein on cell surface and induce apoptosis. Here, we used polystyrene beads coated with CH-11 to investigate the role of lipid rafts in Fas-mediated apoptosis in SKW6.4 cells. Unexpectedly, by treatment of the cells with CH-11-coated beads Fas protein was detached from cell surface and transferred to the surface of CH-11-coated beads. Western blot analysis showed that Fas protein containing both extracellular and intracellular domains was attached to the beads. Fas protein was not transferred from the cells to the surface of the beads coated with other anti-Fas antibodies or Fas ligand. Similar phenomenon was observed in Jurkat T cells. Furthermore, CH-11-induced apoptosis was suppressed by pretreatment with CH-11-coated beads in Jurkat cells. These results suggest that CH-11 might possess distinct properties on Fas protein compared with other anti-Fas antibodies or Fas ligand, and also suggest that caution should be needed to use polystyrene beads coated with antibodies such as CH-11.
机译：小鼠单克隆抗Fas（CD95）抗体克隆CH-11已被广泛用于细胞凋亡的研究。 CH-11具有与细胞表面Fas蛋白结合并诱导凋亡的能力。在这里，我们使用涂有CH-11的聚苯乙烯珠子来研究脂质筏在Fas介导的SKW6.4细胞凋亡中的作用。出乎意料的是，通过用CH-11-包被的珠子处理细胞，Fas蛋白从细胞表面分离并转移到CH-11-包被的珠子表面。蛋白质印迹分析表明，含有细胞外和细胞内结构域的Fas蛋白都附着在磁珠上。 Fas蛋白未从细胞转移到涂有其他抗Fas抗体或Fas配体的珠子表面。在Jurkat T细胞中观察到类似现象。此外，通过用CH-11-包被的珠子预处理Jurkat细胞可抑制CH-11-诱导的细胞凋亡。这些结果表明，与其他抗Fas抗体或Fas配体相比，CH-11在Fas蛋白上可能具有独特的特性，并且还建议使用涂有CH-11等抗体的聚苯乙烯珠时应格外小心。

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