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首页> 外文期刊>European food research and technology =: Zeitschrift fur Lebensmittel-Untersuchung und -Forschung. A >Salicylic acid modulated flavonol biosynthesis in three key phases during grape berry development.
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Salicylic acid modulated flavonol biosynthesis in three key phases during grape berry development.

机译：水杨酸在葡萄浆果发育的三个关键阶段调节黄酮醇的生物合成。

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摘要

Salicylic acid (SA) is a key signaling molecule involved in plant defense responses, former work indicated its regulating effects on some defense genes in plant materials, while the relationship between SA and flavonol biosynthesis during grape berry development remains obscure. By using the techniques of in vivo incubation of the grape berry (Vitis vinifera L. cv. Cabernet Sauvignon) tissues in SA-contained medium, the effects of exogenous SA on flavonol biosynthesis in three key phases (the rapid growth phase, the lag phase of growth and the veraison) of grape berry development were investigated in this work. The results showed that exogenous SA could modulate flavonol biosynthesis, but the modulation effects differed greatly in different phases of grape berry development. During the lag phase of growth and veraison, exogenous SA activated flavonol synthase (FLS) activities and induced the synthesis of new FLS protein. The activation of FLS activity could be blocked by protein synthesis inhibitor cycloheximide and mRNA transcription inhibitor actinomycin D during the lag phase of growth, but only actinomycin D inhibited exogenous SA at veraison. In accordance with the changes of FLS activities and amounts, total flavonol content was found to be modulated accordingly. But the contents of free flavonols had no correlations with SA in the rapid growth phase and veraison. The results suggested that exogenous SA could modulate flavonol biosynthesis of grape berries by activating FLS activity at specific developing phases, while the accumulation of flavonol compounds might be involved in the development of acquired resistance mediated by SA
机译：水杨酸（SA）是参与植物防御反应的关键信号分子，以前的工作表明其对植物材料中某些防御基因的调节作用，而SA和黄酮醇在葡萄浆果发育过程中的生物合成之间的关系仍然不清楚。通过在含SA的培养基中对葡萄浆果（Vitis vinifera L. cv。Cabernet Sauvignon）组织进行体内孵育技术，外源SA对黄酮醇生物合成的三个关键阶段（快速生长阶段，滞后阶段）的影响在这项工作中，研究了葡萄浆果的生长和确证性。结果表明，外源SA可以调节黄酮醇的生物合成，但在葡萄浆果发育的不同阶段，其调节效果差异很大。在生长和验证的滞后阶段，外源SA激活了黄酮醇合酶（FLS）活性并诱导了新FLS蛋白的合成。在生长的滞后阶段，蛋白合成抑制剂环己酰亚胺和mRNA转录抑制剂放线菌素D可以阻止FLS活性的激活，但是只有放线菌素D确实可以抑制外源性SA。根据FLS活性和量的变化，发现总黄酮醇含量被相应地调节。但游离黄酮醇的含量在快速生长期和验证中与SA无关。结果表明外源SA可以通过激活特定发育阶段的FLS活性来调节葡萄浆果的黄酮生物合成，而黄酮化合物的积累可能参与SA介导的获得性抗性的发展。

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