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首页> 外文期刊>European food research and technology =: Zeitschrift fur Lebensmittel-Untersuchung und -Forschung. A >A rapid and high-throughput real-time PCR assay for species identification: application to stockfish sold in Italy
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A rapid and high-throughput real-time PCR assay for species identification: application to stockfish sold in Italy

机译:一种快速,高通量的实时荧光定量PCR检测物种的方法:在意大利出售的stock鱼中的应用

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摘要

In some parts of Italy, there is a tradition of eat-ing special, highly prized species of cod, fished and dried in Norway. In order to safeguard the value of this food product, in 2005, the Italian government legislated that the commercial term "stockfish" can only be attributed to Gadus morhua i Gm). In this study, we present an improved real-time PCR assay for efficient identification of Gm with respect to other gadiforms of commercial interest. The method is applied to 437 stockfish samples, collected in various Italian regions, in order to verify whether labelling regulations had been respected and to report instances of fraud in the Italian stockfish market. The PCR method employed here allowed rapid and economical identification of the species, with a very high percentage of correct identifications.
机译:在意大利的某些地区,有一种吃特殊的,珍贵的鳕鱼的传统,在挪威,它们都经过捕鱼和干燥。为了保护这种食品的价值,意大利政府在2005年立法规定,“干鱼”这一商业术语只能归因于Gadus morhua i Gm。在这项研究中,我们提出了一种改进的实时PCR分析方法,可以有效地鉴定Gm相对于其他商业化的Gadiform。该方法适用于在意大利各个地区收集的437只鱼类样本,以验证是否遵守了标签规定并报告意大利鱼类市场中的欺诈事件。此处使用的PCR方法可以快速,经济地鉴定物种,并且正确鉴定的百分比很高。

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