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首页> 外文期刊>European food research and technology =: Zeitschrift fur Lebensmittel-Untersuchung und -Forschung. A >Genetic system for an integral traceability of European eel (Anguilla anguilla) in aquaculture and seafood products: authentication by fast real-time PCR
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Genetic system for an integral traceability of European eel (Anguilla anguilla) in aquaculture and seafood products: authentication by fast real-time PCR

机译:用于水产养殖和海鲜产品中欧洲鳗(安圭拉鳗)的整体可追溯性的遗传系统:通过快速实时PCR进行鉴定

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摘要

Wild juvenile eels or "glass eels" are caught and used as "seed" in aquaculture production. Eel farming is responsible for over 90 % of all Anguilla production worldwide. Prior to 1990, eel farming was almost exclusively carried out using species of local provenance. However, the exploitation of some species, as A. japonica, has resulted in an increased of eels from European trade, as well as glass eels and other life stages, to supply the aquaculture industry. All eel aquaculture is still based on wild source seed, and this primary resource for aquaculture should be controlled, knowing at all times the origin of the seed that is acquired for farming. Morphological authentication of glass eels is very difficult due to the few diagnostic characters in early stages. For this reason, in the present study a fast real-time PCR methodology has been developed for A. anguilla authentication, Europe's most valuable species. The methodology developed was performed and validated in adult and glass eels. The results show that this methodology is specific and efficient, and it can be apply to adult and glass freshwater eel authentication.
机译:野生的幼鳗或“玻璃鳗”被捕捞并用作水产养殖生产中的“种子”。鳗鱼养殖占全球安圭拉总产量的90%以上。在1990年之前,鳗鱼养殖几乎完全是使用当地种来进行的。然而,对某些物种的开采(如日本粳稻)导致欧洲贸易的鳗鱼以及玻璃鳗鱼和其他生命阶段的数量增加,从而为水产养殖业提供了支持。所有鳗鱼水产养殖仍以野生源种子为基础,这种水产养殖的主要资源应得到控制,并始终了解养殖获得的种子的来源。由于早期阶段的诊断特征很少,玻璃鳗的形态鉴定非常困难。由于这个原因,在本研究中,已经开发了一种快速实时PCR方法,用于欧洲最有价值的物种安圭曲霉的鉴定。所开发的方法已在成年鳗和玻璃鳗中进行并验证。结果表明,该方法具有针对性和有效性,可用于成人和玻璃淡水鳗的认证。

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