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首页> 外文期刊>European Journal of Medicinal Chemistry: Chimie Therapeutique >Hyperhydricity of Prunus avium shoots cultured on gelrite: a controlled stress response
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Hyperhydricity of Prunus avium shoots cultured on gelrite: a controlled stress response

机译：在胶凝体上培养的樱桃李的高水合：可控制的胁迫反应

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摘要

Hyperhydricity is a physiological disorder frequently affecting shoots vegetatively propagated in vitro. Hyperhydric shoots are characterised by a translucent aspect due to a chlorophyll deficiency, a not very developed cell wall and a high water content. Hyperhydricity of Prunus avium shoots was expressed in vitro in one multiplication cycle by replacing the gelling agent agar (normal shoots: NS) by gelrite (hyperhydric shoots: HS). P. avium shoots evolving towards the hyperhydric state produced higher amounts of ethylene, polyamines (PAs) and proline, which are substances considered as stress markers. A higher activity of glutathione peroxidase (GPX; EC 1.11.1.9), involved in organic hydroperoxide elimination, suggested an increased production of these compounds in HS. The unchanged free fatty acid composition indicated no HS membrane damages compared to NS. The ploidy level of HS nuclei was not affected, but the bigger size and the lower percentage of nuclei during the S phase suggested a slowing down of the cell cycle. The results argued for a stress response of the HS, but no signs of oxidative damages of lipid membrane and nucleus were observed. The discussion points out paradoxical results in a classical analysis of stress and suggests an alternative way of defense mechanisms in HS, involving homeostatic regulation and controlled degradation processes to maintain integrity and vital functions of the cell.
机译：体液过高是一种生理性疾病，通常会影响到在体外以营养方式繁殖的枝条。由于叶绿素缺乏，细胞壁不太发达和水含量高，高芽的特点是半透明。在一个繁殖周期中，通过用胶凝剂（水合芽：HS）代替胶凝剂琼脂（正常枝：NS），在一个繁殖周期中表达李属李的高水合性。进化为高羟基态的鸟形假单胞菌芽产生更多量的乙烯，多胺（PAs）和脯氨酸，它们被认为是胁迫标记。谷胱甘肽过氧化物酶（GPX; EC 1.11.1.9）的较高活性参与了有机氢过氧化物的消除，表明这些化合物在HS中的产量增加。与NS相比，未改变的游离脂肪酸组成表明HS膜无损伤。 HS核的倍性水平没有受到影响，但是在S期，较大的核和较低的核百分比提示细胞周期减慢。结果证明了HS的应激反应，但是没有观察到脂质膜和核的氧化损伤的迹象。讨论指出了对压力的经典分析中的悖论结果，并提出了HS中防御机制的另一种方法，其中包括稳态调节和受控降解过程，以维持细胞的完整性和重要功能。

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