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首页> 外文期刊>European Journal of Medicinal Chemistry: Chimie Therapeutique >Light and metabolic regulation of HAS1, HAS1.1 and HAS2, three asparagine synthetase genes in Helianthus annuus
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Light and metabolic regulation of HAS1, HAS1.1 and HAS2, three asparagine synthetase genes in Helianthus annuus

机译：向日葵中三个天冬酰胺合成酶基因HAS1，HAS1.1和HAS2的光和代谢调节

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摘要

The role of light, carbon and nitrogen availability on the regulation of three asparagine synthetase (AS, EC 6.3.5.4)-coding genes, HAS1, HAS1.1 and HAS2, has been investigated in sunflower (Helianthus annuus). The response of each gene to different illumination conditions and to treatments that modify the carbon and nitrogen status of the plant was evaluated by Northern analysis with gene-specific probes. Light represses the expression of HAS1 and HAS1.1. Phytochrome and photosynthesis-derived carbohydrates mediate this repression. On the contrary, maintained HAS2 expression requires light and is positively affected by sucrose. HAS1 and HAS1.1 expression is dependent on nitrogen availability, while HAS2 transcripts are still found in N-starved plants. High ammonium level induces all three AS genes and partially reverts sucrose repression of HAS1 and HAS1.1. In summary, light, carbon and nitrogen availability control asparagine synthesis in sunflower by regulating three AS-coding genes. Illumination and carbon sufficiency maintain HAS2 active to supply asparagine that can be used for growth. Darkness and low C/N ratio conditions trigger the response of the specialized HAS1 and HAS1.1 genes which contribute to store the excess nitrogen as asparagine. Ammonium induces all three AS-genes which may favor its detoxification.
机译：已经研究了向日葵（向日葵）中光，碳和氮的有效性对三种天冬酰胺合成酶（AS，EC 6.3.5.4）编码基因HAS1，HAS1.1和HAS2的调节作用。通过基因特异性探针的Northern分析，评估了每个基因对不同光照条件和改变植物碳和氮状况的处理的反应。光线会抑制HAS1和HAS1.1的表达。植物色素和光合作用衍生的碳水化合物介导这种抑制作用。相反，维持的HAS2表达需要光，并且受到蔗糖的积极影响。 HAS1和HAS1.1的表达取决于氮的有效性，而在缺氮的植物中仍发现HAS2转录本。高铵水平会诱导所有三个AS基因，并部分还原蔗糖阻抑HAS1和HAS1.1。总之，光，碳和氮的可利用性通过调节三个AS编码基因来控制向日葵中的天冬酰胺合成。光照和碳充足性使HAS2保持活性，可提供可用于生长的天冬酰胺。黑暗和低C / N比条件会触发专门的HAS1和HAS1.1基因的响应，这些基因有助于存储过量的天冬酰胺氮。铵诱导所有三个AS基因，这可能有利于其解毒。

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