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首页> 外文期刊>European Journal of Medicinal Chemistry: Chimie Therapeutique >Photoperiod and temperature effects on in vitro growth and flowering of P. pusilla, an epiphytic orchid
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Photoperiod and temperature effects on in vitro growth and flowering of P. pusilla, an epiphytic orchid

机译：光周期和温度对附生兰花P. pusilla体外生长和开花的影响

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摘要

Psygmorchis pusilla Dodson and Dressler, an epiphytic orchid, has been shown to be an interesting model to study in vitro flower formation. In the present study, the effects of photoperiod and temperature on vegetative and reproductive development were investigated. Although photoperiod had limited effects on leaf number, an etiolating process was verified in darkness and a higher growth was detected under long days. A positive relationship was observed between long days and floral spike formation. However, plant incubation under 20 h photoperiod or longer days negatively affected floral bud development, inhibiting anthesis and reducing flower longevity. Higher soluble sugar and starch levels were detected in plants cultivated under long days, while chlorophyll and carotenoids contents were negatively affected under these conditions. Plants showed great sensitivity to temperature variations; 27 °C being the most adequate for growth, leaf and floral spike formation. Temperatures of 22 and 32 °C were not appropriate for in vitro development of P. pusilla.
机译：Psygmorchis pusilla Dodson和Dressler，一种附生兰花，已被证明是研究体外花形成的有趣模型。在本研究中，研究了光周期和温度对营养和生殖发育的影响。尽管光周期对叶片数的影响有限，但在黑暗中验证了黄化过程，并且在长日照下检测到较高的生长。在长日和花穗形成之间观察到正相关。但是，在20 h或更长的光照时间下进行植物培养会对花蕾的发育产生负面影响，从而抑制花药的生长并降低花的寿命。在长时间种植的植物中检测到较高的可溶性糖和淀粉水平，而在这些条件下叶绿素和类胡萝卜素含量受到负面影响。植物对温度变化表现出极大的敏感性。 27°C最适合生长，叶片和花穗形成。 22°C和32°C的温度不适用于脓疱丙酸杆菌的体外发育。

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