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首页> 外文期刊>European Journal of Medicinal Chemistry: Chimie Therapeutique >Spread of oxidative damage and antioxidative response through cell layers of tobacco callus after UV-C treatment
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Spread of oxidative damage and antioxidative response through cell layers of tobacco callus after UV-C treatment

机译：UV-C处理后通过烟草愈伤组织细胞层传播氧化损伤和抗氧化反应

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摘要

Tobacco (Nicotiana tabacum L. cv. Petit Havana) callus cultures were exposed to UV-C high dose pulse-treatment (254 nm, 50 kJ m~(–2), 1 h-treatment). After 6, 24 and 48 h from the end of the treatment, calli were cut transversally in two layers and oxidative damage (malondialdehyde [MDA] and hydrogen peroxide), non-enzymatic (radical scavenging antioxidants [RSA] and polyamines) and enzymatic antioxidants (ascorbate peroxidase [APX, EC 1.11.1.11], glutathione reductase [GR, EC 1.6.4.2], catalase [CAT, EC 1.11.1.6] and guaiacol peroxidase [GPX, EC 1.11.1.7]) were evaluated. At each time-point data referred to UV-C treated calli were compared to data of untreated ones (control). Despite of a strong increase of H2O2 content, a slight cellular damage was observed in both upper and lower layers 24 and 48 h after UV-C treatment. An activation first of non-enzymatic antioxidants and then of enzymatic antioxidants was detected in UV-C treated calli. In particular, RSA and putrescine (PUT) accumulated 6 h after UV-C treatment while APX, GR and GPX enzyme activities increased 24 h after UV-C irradiation. Catalase activity did not change. UV-C-induced oxidative stress and antioxidative response were observed also in cell layers not directly exposed to UV irradiation, indicating that a stress signal was transmitted to the whole mass of callus.
机译：烟草（烟草（Nicotiana tabacum L. cv。Petit Havana））的愈伤组织培养物经过UV-C高剂量脉冲处理（254 nm，50 kJ m〜（–2），1 h处理）。处理结束后的6、24和48小时后，将愈伤组织横向切成两层，并氧化破坏（丙二醛[MDA]和过氧化氢），非酶促（自由基清除抗氧化剂[RSA]和多胺）和酶促抗氧化剂（评估了抗坏血酸过氧化物酶[APX，EC 1.11.1.11]，谷胱甘肽还原酶[GR，EC 1.6.4.2]，过氧化氢酶[CAT，EC 1.11.1.6]和愈创木酚过氧化物酶[GPX，EC 1.11.1.7]）。在每个时间点，将经过UV-C处理的愈伤组织的数据与未经处理的愈伤组织的数据进行比较（对照）。尽管H2O2含量大大增加，但在UV-C处理后24和48 h在上层和下层均观察到轻微的细胞损伤。在UV-C处理的愈伤组织中，首先检测到非酶抗氧化剂的激活，然后是酶抗氧化剂的激活。特别是，UV-C处理后6小时，RSA和腐胺（PUT）积累，而UV-C照射后24小时，APX，GR和GPX酶活性增加。过氧化氢酶活性没有改变。在未直接暴露于紫外线辐射的细胞层中也观察到了UV-C诱导的氧化应激和抗氧化反应，表明应激信号已传递至整个愈伤组织。

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