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首页> 外文期刊>European Journal of Medicinal Chemistry: Chimie Therapeutique >Biochemical characterization of a high-palmitoleic acid Helianthus annuus mutant
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Biochemical characterization of a high-palmitoleic acid Helianthus annuus mutant

机译：高棕榈油酸向日葵突变体的生化特性

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摘要

In the present work we carried out analytical and biochemical studies on a new high-n-7 monounsaturated fatty acid sunflower (Helianthus annuus L.) mutant. This new line, which has been selected by classical methods of breeding and mutagenesis, shows contents of unusual acyl chains up to 20% (12% of 16:1~(Δ9), 5% of 16:2~(Δ9,12) and 6% of 18:1~(Δ11)), whereas those fatty acids are found in negligible amounts in common sunflower cultivars. This characterization involved in vivo incubations with radiolabeled acetate and measurement of the last enzymes involved in the intraplastidial de novo fatty acid synthesis: β-ketoacyl-ACP synthase II, stearoyl-ACP desaturase (EC 1.14.19.2) and acyl-ACP thioesterases (EC 3.1.2.14). Results indicated that the high-palmitoleic acid phenotype was associated with a concerted reduction in the fatty acid synthase II activity with respect to the control lines and an increase of stearoyl-ACP desaturase activity with respect to the high-palmitate mutant line.
机译：在当前的工作中，我们对一个新的高n-7单不饱和脂肪酸向日葵（Helianthus annuus L.）突变体进行了分析和生化研究。通过经典育种和诱变方法选择的新品系，其异常酰基链含量高达20％（16：1〜（Δ9）的12％，16：2〜（Δ9,12）的5％）和18：1〜（Δ11）的6％，而在普通向日葵品种中发现的脂肪酸含量可忽略不计。该表征涉及放射性标记的乙酸盐在体内的孵育以及质体内从头脂肪酸合成中涉及的最后酶的测量：β-酮酰基-ACP合酶II，硬脂酰-ACP去饱和酶（EC 1.14.19.2）和酰基-ACP硫酯酶（EC 3.1.2.14）。结果表明，相对于对照品系，高棕榈油酸表型与脂肪酸合酶II活性的一致降低有关，与相对于高棕榈酸酯化的突变株系的硬脂酰-ACP去饱和酶活性的增加相关。

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