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首页> 外文期刊>European Journal of Medicinal Chemistry: Chimie Therapeutique >Fluorescence spectroscopic investigation of the interaction between chloramphenicol and lysozyme.
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Fluorescence spectroscopic investigation of the interaction between chloramphenicol and lysozyme.

机译：氯霉素与溶菌酶之间相互作用的荧光光谱研究。

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摘要

The interactions between chloramphenicol and lysozyme were studied using fluorescence, UV/vis and circular dichroism spectra. The results proved the mechanism of fluorescence quenching of lysozyme by chloramphenicol is due to the formation of lysozyme-chloramphenicol complex. The thermodynamic parameters, enthalpy change (DeltaH) and entropy change (DeltaS) for the reaction, were calculated to be -12.41 kJ mol(-1) and 37.99 J mol(-1)K(-1), which indicated that hydrophobic force and hydrogen bond were the dominant intermolecular forces in stabilizing the complex. The distance r=3.99 nm between donor and acceptor was obtained according to Forster's theory. In addition, the alterations of lysozyme secondary structure in the presence of chloramphenicol were confirmed by the evidences from circular dichroism, synchronous and three-dimensional fluorescence spectroscopy.
机译：使用荧光，紫外/可见光和圆二色性光谱研究了氯霉素和溶菌酶之间的相互作用。结果证明氯霉素对溶菌酶进行荧光猝灭的机理是由于溶菌酶-氯霉素复合物的形成。反应的热力学参数，焓变（DeltaH）和熵变（DeltaS）计算为-12.41 kJ mol（-1）和37.99 J mol（-1）K（-1），表明疏水力氢键和氢键是稳定该配合物的主要分子间力。根据Forster理论获得供体与受体之间的距离r ＝ 3.99nm。另外，通过圆二色性，同步和三维荧光光谱的证据证实了在氯霉素存在下溶菌酶二级结构的改变。

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