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首页> 外文期刊>European Journal of Medicinal Chemistry: Chimie Therapeutique >The role of lipoic acid in the regulation of the redox status of wheat irrigated with 20% sea water
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The role of lipoic acid in the regulation of the redox status of wheat irrigated with 20% sea water

机译：硫辛酸在调节20％海水灌溉的小麦的氧化还原状态中的作用

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The effect of irrigation with 20% sea water was studied in 14 and 21-day-old seedlings of durum wheat (Triticum durum, cv. Ofanto). Comparisons between control (Hoagland’s 2 solution) and treated (20% sea water in Hoagland’s solution) plants included, besides HPLC determination of reduced (DHLA) and oxidised (LA) forms of lipoic acid, ascorbate and glutathione contents, their redox status, the activity of ascorbate peroxidase (APX, EC 1.11.1.11.) and glutathione reductase (GR, EC 1.6.4.2.). The results indicated a more relevant presence of lipoic acid in the roots in comparison to the shoots. An involvement of its reduced form in the regeneration of the reduced glutathione, at least at 14 days of treatment, suggested, besides its role as dehydrogenase enzyme cofactor, a role in the recycling of the other antioxidants. The amount of LA always increased with growth in shoots and decreased in roots, while DHLA remained constant in control and increased in treated plants. Besides, the oxidised form always decreased with sea water while the reduced form decreased in shoots and increased in roots. The ascorbate pool exerted its positive influence especially in the shoots, while APX and GR activities resulted differently modulated by the salinity level.
机译：在硬质小麦（Triticum durum，cv。Ofanto）的14和21天大的幼苗中研究了用20％海水灌溉的效果。对照植物（Hoagland's 2溶液）和处理过的植物（Hoagland's溶液中的20％海水）之间的比较，除了HPLC测定硫辛酸，抗坏血酸和谷胱甘肽含量的还原（DHLA）和氧化（LA）形式，其氧化还原状态，抗坏血酸过氧化物酶（APX，EC 1.11.1.11。）和谷胱甘肽还原酶（GR，EC 1.6.4.2。）的活性。结果表明，与芽相比，硫辛酸在根中的存在更为相关。至少在治疗第14天，其还原形式参与了还原型谷胱甘肽的再生，这表明它除了具有脱氢酶辅因子的作用外，还具有其他抗氧化剂再循环的作用。 LA的量总是随着芽的生长而增加，而根却减少，而DHLA在对照中保持恒定，而在处理过的植物中则增加。此外，氧化态总是随海水而减少，而还原态在芽中减少而在根中增加。抗坏血酸池发挥了积极的影响，特别是在芽中，而APX和GR的活性受到盐度水平的不同调节。

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