• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>European Journal of Medicinal Chemistry: Chimie Therapeutique >Larvicidal Cry proteins from Bacillus thuringiensis are released in root exudates of transgenic B. thuringiensis corn, potato, and rice but not of B. thuringiensis canola, cotton, and tobacco
【24h】

Larvicidal Cry proteins from Bacillus thuringiensis are released in root exudates of transgenic B. thuringiensis corn, potato, and rice but not of B. thuringiensis canola, cotton, and tobacco

机译：苏云金芽孢杆菌的杀幼虫Cry蛋白在转基因苏云金芽孢杆菌的玉米，马铃薯和大米的根分泌物中释放，而在苏云金芽孢杆菌的双低油菜籽，棉花和烟草中不释放

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Larvicidal proteins encoded by cry genes from Bacillus thuringiensis were released in root exudates from transgenic B. thuringiensis corn, rice, and potato but not from B. thuringiensis canola, cotton, and tobacco. Nonsterile soil and sterile hydroponic solution in which B. thuringiensis corn, rice, or potato had been grown were immunologically positive for the presence of the Cry proteins; from B. thuringiensis corn and rice, the soil and solution were toxic to the larva of the tobacco hornworm (Manduca sexta), and from potato, to the larva of the Colorado potato beetle (Leptinotarsa decemlineata), representative lepidoptera and coleoptera, respectively. No toxin was detected immunologically or by larvicidal assay in soil or hydroponic solution in which B. thuringiensis canola, cotton, or tobacco, as well as all near-isogenic non-B. thuringiensis plant counterparts or no plants, had been grown. All plant species had the cauliflower mosaic virus (CaMV) 35S promoter, except rice, which had the ubiquitin promoter from maize. The reasons for the differences between species in the exudation from roots of the toxins are not known. The released toxins persisted in soil as the result of their binding on surface-active particles (e.g. clay minerals, humic substances), which reduced their biodegradation. The release of the toxins in root exudates could enhance the control of target insect pests, constitute a hazard to nontarget organisms, and/or increase the selection of toxin-resistant target insects.
机译：苏云金芽孢杆菌的cry基因编码的杀幼虫蛋白在苏云金芽孢杆菌的玉米，水稻和马铃薯的根分泌物中释放，而苏云金芽孢杆菌的双低油菜籽，棉花和烟草则没有。生长有苏云金芽孢杆菌玉米，水稻或马铃薯的非无菌土壤和无菌水培溶液，对于Cry蛋白的存在在免疫学上呈阳性。从苏云金芽孢杆菌玉米和水稻中提取的土壤和溶液对烟草天蛾幼虫（Manduca sexta）有毒，从马铃薯到科罗拉多马铃薯甲虫（Leptinotarsa decemlineata）的幼虫有毒，分别代表鳞翅目和鞘翅目。在苏云金芽孢杆菌低芥酸菜籽，棉花或烟草以及所有近等基因的非B的土壤或水培溶液中，未通过免疫学或通过杀幼虫剂检测到毒素。已经生长了苏云金植物的对应物或没有植物。除水稻外，所有植物物种均具有花椰菜花叶病毒（CaMV）35S启动子，而水稻具有玉米的遍在蛋白启动子。从毒素的根部渗出的种间差异的原因尚不清楚。释放的毒素由于与表面活性颗粒（例如粘土矿物，腐殖质）结合而保留在土壤中，从而降低了其生物降解能力。毒素在根系分泌物中的释放可增强对目标害虫的控制，对非目标生物构成危害，和/或增加抗毒素目标昆虫的选择。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号