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首页> 外文期刊>European Journal of Medicinal Chemistry: Chimie Therapeutique >Programmed cell death induces male sterility in Actinidia deliciosa female flowers
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Programmed cell death induces male sterility in Actinidia deliciosa female flowers

机译：程序性细胞死亡诱导猕猴桃雌花中的雄性不育

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摘要

The importance of programmed cell death (PCD) during the life cycle of plants is well established, although the underlying molecular mechanisms are still poorly defined. An emerging system for the study of PCD during development in plants is that of sex organ abortion. In this work we investigate the degeneration of microspores in the anthers of Actinidia deliciosa female flowers. The kiwifruit, A. deliciosa, is a dioecious species native to China. Pollen development in female flowers is equivalent to pollen development in the male flowers, until the microspores are released from the tetrads. At this time the first differences appear, and include the condensation and shrinkage of the cytoplasm, blebbing of the plasma membrane and of the nuclear envelope, and condensation of chromatin. However, at the time these events are occurring, all other cellular organelles, including mitochondria, have their structures well preserved. Fragmentation of DNA was detected in situ by the TUNEL procedure, which involves the end labeling of the DNA fragments by terminal deoxynucleotidyl transferase with UTP conjugated to a detectable marker. This assay confirmed the morphological characterization of PCD in this system.
机译：尽管在潜在的分子机制中仍未明确定义，但在植物生命周期中程序性细胞死亡（PCD）的重要性已得到充分确立。一种在植物发育过程中研究PCD的新兴系统是性器官流产系统。在这项工作中，我们调查了猕猴桃雌花的花药中小孢子的退化。奇异果A. deliciosa是中国特有的雌雄异株。雌花中的花粉发育等同于雄花中的花粉发育，直到小孢子从四分体中释放出来。这时出现第一个差异，包括细胞质的凝结和收缩，质膜和核被膜的起泡以及染色质的凝结。但是，在这些事件发生时，所有其他细胞器（包括线粒体）的结构都得到了很好的保存。通过TUNEL程序原位检测DNA片段化，该过程涉及通过末端脱氧核苷酸转移酶与结合到可检测标记上的UTP末端标记DNA片段。该测定法证实了该系统中PCD的形态特征。

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