...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioanalysis >The advantages of microflow LC-MS/MS compared with conventional HPLC-MS/MS for the analysis of methotrexate from human plasma
【24h】

The advantages of microflow LC-MS/MS compared with conventional HPLC-MS/MS for the analysis of methotrexate from human plasma

机译：与常规HPLC-MS / MS相比，微流LC-MS / MS在分析人血浆中的甲氨蝶呤方面的优势

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: In support of bioanalysis, there has always been a desire to improve detection limits and reduce scale. Microflow LC (MFLC) coupled with MS accomplishes both of these goals. Results: As such, MFLC coupled with an MS system was used to generate bioanalytical validation data that met US FDA criteria. The MFLC-MS/MS data was compared with the same method with the use of conventional HPLC-MS/MS and a more than 14× S/N improvement was found with the MFLC-MS/MS method. Methotrexate was used as a model molecule to demonstrate the validation of the method from human plasma. The MFLC-MS/MS method was demonstrated to be accurate (±7%) and precise (12.9% at the LLOQ and a maximum of 11.6% at all other concentrations) across the dynamic range of the assay (1-1000 ng/ml) and compared well with the HPLC-MS/MS method. The MFLC bioanalytical validation was performed at a flow rate of 35 μl/min on a 0.5-mm inner diameter (I.D.) column, whereas, for the same linear velocities on the 2.0-mm I.D. column, the conventional HPLC bioanalytical validation was performed at 700 μl/min. Since the flow rate of the MFLC system is 20-times less than the HPLC system, the consumable solvent and disposal cost to perform the MFLC validation was significantly less. Conclusion: MFLC-MS/MS can be used to perform bioanalytical method validations with increased MS signal, reduced source contamination and reduced solvent consumption.
机译：背景：为了支持生物分析，一直希望提高检测限并缩小规模。 Microflow LC（MFLC）与MS结合可实现这两个目标。结果：这样，MFLC与MS系统结合用于生成符合美国FDA标准的生物分析验证数据。 MFLC-MS / MS数据与使用常规HPLC-MS / MS的相同方法进行了比较，发现MFLC-MS / MS方法的S / N改善了14倍以上。甲氨蝶呤用作模型分子，以从人血浆中验证该方法的有效性。在整个测定动态范围（1-1000 ng / ml）中，MFLC-MS / MS方法被证明是准确的（±7％）和精确的（LLOQ为12.9％，所有其他浓度的最大值为11.6％） ），并与HPLC-MS / MS方法比较。在0.5毫米内径（I.D.）色谱柱上以35μl/ min的流速进行MFLC生物分析验证，而在2.0毫米I.D.上以相同的线速度进行。色谱柱中，常规HPLC生物分析验证以700μl/ min进行。由于MFLC系统的流速比HPLC系统小20倍，因此执行MFLC验证所需的溶剂和处理成本大大降低。结论：MFLC-MS / MS可用于进行生物分析方法验证，同时增加MS信号，减少源污染和减少溶剂消耗。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号