...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioanalysis >Evaluation of 5-methyl-2′-deoxycytidine stability in hydrolyzed and nonhydrolyzed DNA by HPLC-UV
【24h】

Evaluation of 5-methyl-2′-deoxycytidine stability in hydrolyzed and nonhydrolyzed DNA by HPLC-UV

机译：高效液相色谱-紫外光谱法评估水解和未水解DNA中5-甲基-2'-脱氧胞苷的稳定性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Although the determination of 5-methyl-2′-deoxycytidine (5-MedC) in various biological samples is gaining increasing scientific interest, there are no data available regarding its stability. Results: We have currently evaluated the stability of 5-MedC and 2′-deoxycytidine (dC) at -20°C, both in hydrolyzed and nonhydrolyzed calf thymus DNA (CT DNA), as well as following repetitive freeze-thaw cycles. HPLC-UV was used for the accurate determination of the two 2′-deoxynucleosides. Statistical evaluation of the results revealed that 5-MedC and dC were stable in hydrolyzed CT DNA for at least 7 days and in nonhydrolyzed CT DNA for at least 65 days, when these were stored at -20°C. Furthermore, both 2′-deoxynucleosides were stable for at least three repetitive freeze-thaw cycles. Conclusion: By using HPLC-UV, we have evaluated the stability of 5-MedC and dC under storage conditions and repetitive freeze-thaw cycles. Our results are informative about the way samples should be handled and stored in epigenetic studies.
机译：背景：尽管测定各种生物样品中的5-甲基-2'-脱氧胞苷（5-MedC）越来越引起科学兴趣，但尚无关于其稳定性的数据。结果：我们目前已评估了在-20°C下5-MedC和2'-脱氧胞苷（dC）在水解和未水解小牛胸腺DNA（CT DNA）中以及在重复的冻融循环后的稳定性。 HPLC-UV用于准确测定两个2'-脱氧核苷。结果的统计评估表明，当将它们存储在-20°C时，5-MedC和dC在水解的CT DNA中稳定至少7天，在非水解的CT DNA中稳定至少65天。此外，两个2'-脱氧核苷在至少三个重复的冻融循环中均稳定。结论：通过HPLC-UV，我们评估了5-MedC和dC在储存条件和重复冻融循环下的稳定性。我们的结果提供了有关表观遗传研究中样品处理和储存方式的信息。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号