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首页> 外文期刊>European Journal of Cell Biology: Journal of Deutsche Gesellschaft fur Elektronenmikroskopie: Journal of Deutsche Gesellschaft fur Zellbiologie >abLIM3 is a novel component of adherens junctions with actin-binding activity
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abLIM3 is a novel component of adherens junctions with actin-binding activity

机译：abLIM3是具有肌动蛋白结合活性的粘附连接的新型成分

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摘要

The interactions of adhesion molecules with dense actin filaments via cytoplasmic plaque proteins are crucial for the adhesive function of adherens junctions (AJs) in epithelial and endothelial cells. Using localization-based expression cloning, we identified abLIM3, a member of the actin-binding LIM (abLIM) protein family, as a component of the junctional complex. Immunolocalization studies revealed that abLIM3 was localized at AJs in limited cell types, including hepatocytes, bronchial epithelial cells, mesothelial cells and endothelial cells lining muscular tissues. Deletion mutant analyses in cultured cells showed that the C-terminal dematin-like domain of abLIM3, which bound to actin filaments in vitro, was colocalized with phalloidin-stained filamentous actin, whereas the N-terminal LIM domains of abLIM3 were sufficient for recruitment to cell-cell contacts. These results suggest that abLIM3 is involved in anchoring LIM domain-binding components of AJs to circumferential actin bundles in specific cell types
机译：粘附分子通过细胞质斑蛋白与致密肌动蛋白丝的相互作用对于上皮细胞和内皮细胞中粘附连接（AJs）的粘附功能至关重要。使用基于本地化的表达克隆，我们鉴定了肌动蛋白结合LIM（abLIM）蛋白家族成员abLIM3，作为连接复合物的组成部分。免疫定位研究表明，abLIM3定位于AJs中的有限细胞类型中，包括肝细胞，支气管上皮细胞，间皮细胞和衬在肌肉组织中的内皮细胞。在培养细胞中的缺失突变体分析显示，与肌动蛋白丝结合的abLIM3的C端类脱铁蛋白样结构域与鬼笔环素染色的丝状肌动蛋白共定位，而abLIM3的N端LIM结构域足以招募到细胞间接触。这些结果表明，abLIM3参与将AJs的LIM结构域结合成分锚定至特定细胞类型中的圆周肌动蛋白束

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