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首页> 外文期刊>European Journal of Cell Biology: Journal of Deutsche Gesellschaft fur Elektronenmikroskopie: Journal of Deutsche Gesellschaft fur Zellbiologie >Mutants of the Saccharomyces cerevisiae VPS genes CCZ1 and YPT7 are blocked in different stages of sporulation
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Mutants of the Saccharomyces cerevisiae VPS genes CCZ1 and YPT7 are blocked in different stages of sporulation

机译：酿酒酵母VPS基因CCZ1和YPT7的突变体在孢子形成的不同阶段被阻断

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摘要

The CCZ1 gene is a member of the class B VPS (vacuolar protein sorting) genes and it is engaged in the last stage of delivery of multiple kinds of cargo to the yeast vacuole. In the process of fusion of the multivesicular body (MVB) with the vacuole, Ccz1p forms a complex with Ypt7p. Both genes are nonessential for vegetative growth, but their deletions cause a complete block in spore formation. The results of this study indicate that ccz1 Delta cells initiate the meiotic program, properly proceed through premeiotic DNA replication and through the pairing of homologous chromosomes, but fail to progress through the first meiotic divisions and arrest in prophase I with a single nucleus. The mutant cells are defective in spindle formation as well as in duplication and/or separation of the SPBs. ypt7 Delta cells, on the other hand, cannot execute DNA synthesis. We also show that expression of a mutated variant of the YPT7 gene suppresses the sporulation and autophagy defects of ccz1 Delta cells to a quantitatively similar level, suggesting that restoration of autophagy in the ccz1 Delta strain is sufficient to enable its sporulation
机译：CCZ1基因是B类VPS（真空蛋白分选）基因的成员，它参与将多种货物输送到酵母液泡的最后阶段。在多泡体（MVB）与液泡融合的过程中，Ccz1p与Ypt7p形成复合物。这两个基因对于营养生长都不重要，但是它们的缺失会导致孢子形成的完全阻断。这项研究的结果表明ccz1 Delta细胞可以启动减数分裂程序，通过减数分裂前的DNA复制和同源染色体的配对而正确进行，但无法通过第一个减数分裂而继续前进，并停留在前期的单核中。突变细胞在纺锤体形成以及SPB的复制和/或分离方面均存在缺陷。另一方面，ypt7 Delta细胞无法执行DNA合成。我们还显示，YPT7基因突变变体的表达将ccz1 Delta细胞的孢子形成和自噬缺陷抑制到定量相似的水平，这表明ccz1 Delta菌株中自噬的恢复足以使其形成孢子

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