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首页> 外文期刊>European Journal of Cell Biology: Journal of Deutsche Gesellschaft fur Elektronenmikroskopie: Journal of Deutsche Gesellschaft fur Zellbiologie >Actin microfilaments are essential for the cytological positioning andmorphology of the Golgi complex
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Actin microfilaments are essential for the cytological positioning andmorphology of the Golgi complex

机译：肌动蛋白微丝对于高尔基复合体的细胞学定位和形态至关重要

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The organization and function of the Golgi complex was studied in normal rat kidney cells following disruption of the actin cytoskeleton induced by cytochalasin D. In cells treated with these reagents, the reticular and perinuclear Golgi morphology acquired a cluster shape restricted to the centrosome region. Golgi complex alteration affected all Golgi subcompartments as revealed by double fluorescence staining with antibodies to the cis/middle Mannosidase II and the trans-Golgi network TGN38 proteins or vital staining with the lipid derivate C-6-NBD-ceramide. The ultrastructural and stereological analysis showed that the Golgi cisternae remained attached in a stacked conformation, but they were swollen and contained electron-dense intra-cisternal bodies, The Golgi complex cluster remained linked to microtubules since it was fragmented and dispersed after treatment with nocodazole. Moreover, the reassembly of Golgi fragments after the disruption of the microtubuli with nocodazole does not utilize the actin microfilaments. The actin microfilament requirement for the disassembly and reassembly of the Golgi complex and for the ER-Golgi vesicular transport were also studied. The results show that actin microfilaments are not needed for either the retrograde fusion of the Golgi complex with the endoplasmic reticulum promoted by brefeldin A or the anterograde reassembly after the removal of the drug, or the ER-Golgi transport of VSV-G glycoprotein. However, actin microfilaments are directly involved in the subcellular localization and the morphology of the Golgi complex.
机译：在细胞松弛素D诱导的肌动蛋白细胞骨架破裂后，在正常的大鼠肾脏细胞中研究了高尔基复合体的组织和功能。在用这些试剂处理的细胞中，网状和核高尔基体形态获得了局限于中心体区域的簇状。高尔基体改变影响了所有高尔基体的小室，这是通过对顺/中间甘露糖苷酶II和反式高尔基体网络TGN38蛋白的抗体进行的双重荧光染色或用脂质衍生的C-6-NBD-神经酰胺进行的活体染色来揭示的。超微结构和立体分析表明，高尔基水箱保持堆叠状态，但肿胀并含有电子致密的脑池内体。高尔基复合物簇由于与诺考达唑处理后被破碎和分散，因此仍与微管连接。而且，在用诺考达唑破坏微管后，高尔基体片段的重新组装没有利用肌动蛋白微丝。还研究了肌动蛋白微丝对高尔基复合体的拆卸和重新组装以及ER-高尔基囊泡运输的要求。结果表明肌动蛋白微丝既不需要高尔基复合体与布雷菲德菌素A促进的内质网的逆行融合，也不需要去除药物后的顺行重组，也不需要ER-高尔基体运输VSV-G糖蛋白。然而，肌动蛋白微丝直接参与高尔基复合体的亚细胞定位和形态。

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