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首页> 外文期刊>European Journal of Cell Biology: Journal of Deutsche Gesellschaft fur Elektronenmikroskopie: Journal of Deutsche Gesellschaft fur Zellbiologie >Traffic into the prevacuolar/endosomal compartment of Saccharomycescerevisiae: A VPS45-dependent intracellular route and a VPS45-independent,endocytic route
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Traffic into the prevacuolar/endosomal compartment of Saccharomycescerevisiae: A VPS45-dependent intracellular route and a VPS45-independent,endocytic route

机译：进入酿酒酵母前腔室/内膜腔室的运输：依赖VPS45的细胞内途径和不依赖VPS45的内吞途径

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摘要

The vps (vacuolar protein sorting) mutants have been used to dissect and charaterize the vacuolar biogenesis pathway in the yeast Saccharomyces cerevisiae. The vps mutants were isolated through their loss of ability to correctly sort the vacuolar hydrolase CPY, which travels from Golgi membranes to the vacuole through a prevacuolar compartment, Over 50 VPS genes have been divided into 6 classes according to vacuolar morphology, Mutations in any one of the class E VPS genes, such as VPS27, lead to an exaggerated form of the prevacuolar compartment. This class E compartment contains endocytosed proteins as well as proteins en route to the vacuole, and is thus taken to represent an intersection point between the endocytic and biosynthetic pathways. Mutations in the class D gene VPS45 can be used to define a second transport intermediate along the vacuolar biogenesis pathway, Golgi-derived transport vesicles carrying vacuolar membrane proteins on their way to the vacuole, Here we demonstrate that the Sec1p-like protein Vps45p is required for the fusion of Golgi-derived vesicles with the prevacuolar compartment indicating that VPS45 functions before VPS27 in the vacuolar biogenesis pathway, In addition, we show that VPS45 function is not required for the delivery of endocytosed proteins to the prevacuolar compartment from the plasma membrane suggesting that the function of Vps45p is restricted to a single vesicular pathway.
机译：vps（真空蛋白分选）突变体已用于解剖和鉴定啤酒酵母中的液泡生物发生途径。分离了vps突变体，因为它们失去了正确分类液泡水解酶CPY的能力，CPY从高尔基膜穿过液泡前腔进入液泡。根据液泡形态将50多个VPS基因分为6类，任何一种突变E类VPS基因例如VPS27的表达导致前泡区室的夸大形式。这种E类隔室包含内吞蛋白以及到达液泡的蛋白，因此被视为代表内吞和生物合成途径之间的交点。 D类基因VPS45中的突变可用于定义沿液泡生物发生途径的第二种转运中间体，高尔基衍生的转运泡在液泡途中携带液泡膜蛋白，此处我们证明需要Sec1p样蛋白Vps45p高尔基体囊泡与液泡前腔室的融合，表明VPS45在液泡生物发生途径中先于VPS27起作用，此外，我们表明VPS45功能对于将胞吞蛋白从质膜传递至液泡前腔室不是必需的，提示Vps45p的功能仅限于单个囊泡途径。

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