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首页> 外文期刊>European Journal of Cell Biology: Journal of Deutsche Gesellschaft fur Elektronenmikroskopie: Journal of Deutsche Gesellschaft fur Zellbiologie >Caulerpenyne interferes with microtubule-dependent events during the firstmitotic cycle of sea urchin eggs
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Caulerpenyne interferes with microtubule-dependent events during the firstmitotic cycle of sea urchin eggs

机译：Caulerpenyne在海胆卵的有丝分裂周期中干扰微管依赖性事件

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摘要

Caulerpenyne (Cyn), the major secondary metabolite synthesized by the green alga Caulerpa taxifolia proliferating in the Mediterranean Sea, is a cytotoxic sesquiterpene. As this compound has an antiproliferative potency by inhibiting division of many types of cells, we examined the precise effects of Cyn during the early development of the sea urchin Paracentrotus lividus. Whereas Cyn (60 mu M) had no effect on fertilization, it blocked the first cell division in the same manner whether added before or after fertilization, provided the drug was added before or during metaphase, Immunofluorescence localization revealed that Cyn had no effect on the microtubular sperm aster formation, pronuclei migration and fusion, chromosome condensation, nuclear envelope breakdown, and bipolar mitotic spindle assembly. However, mitosis was blocked in a metaphase-like stage at which most chromosomes were aligned at the equatorial plate, while a few of them had not even migrated towards the metaphase plate, When added after the metaphase-anaphase transition, the first division occurred normally but the second division was inhibited with the same phenotype as described above, We previously showed that Cyn did not affect protein synthesis or H1 kinase activation or deactivation (Pesando et al,, 1996, Aquat, Toxicol, 35, 139), but that it partially inhibited DNA synthesis. Our results establish that Cyn does not affect the microfilament-dependent processes of fertilization and cytokinesis and allows the beginning of mitosis, but prevents normal DNA replication and results in metaphase-like arrest of sea urchin embryos.
机译：Caulerpenyne（Cyn）是由绿藻Caulerpa taxifolia在地中海中繁殖的主要次要代谢产物，是一种具有细胞毒性的倍半萜。由于该化合物通过抑制多种类型的细胞分裂而具有抗增殖能力，因此我们在海胆Paracentrotus lividus的早期发育过程中研究了Cyn的精确作用。尽管Cyn（60μM）对受精没有影响，但无论在受精之前还是之后，都以相同的方式阻止了第一次细胞分裂，但前提是在中期之前或中期添加了该药物，免疫荧光定位表明Cyn对受精没有影响。微管精子紫formation形成，前核迁移和融合，染色体凝结，核膜破裂和双极有丝分裂纺锤体组装。然而，有丝分裂在中期样阶段被阻止，在该阶段大多数染色体在赤道板上排列，而其中一些甚至没有迁移到中期板。在中期-后期过渡后添加时，第一次分裂正常发生但是第二个分裂却受到与上述相同的表型的抑制。我们先前证明Cyn不会影响蛋白质合成或H1激酶的激活或失活（Pesando等人，1996，Aquat，Toxicol，35，139），但是部分抑制DNA合成。我们的研究结果表明，Cyn不会影响微丝依赖的受精和胞质分裂过程，并允许有丝分裂的开始，但会阻止正常的DNA复制，并导致海胆胚胎的中期样停滞。

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