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首页> 外文期刊>European Journal of Cell Biology: Journal of Deutsche Gesellschaft fur Elektronenmikroskopie: Journal of Deutsche Gesellschaft fur Zellbiologie >Cytoplasmic intermediate filament protein expression in tunicate development: a specific marker for the test cells
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Cytoplasmic intermediate filament protein expression in tunicate development: a specific marker for the test cells

机译：被膜发育中的细胞质中间丝蛋白表达：受试细胞的特异性标记

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The urochordate Ciona intestinalis is a well established system for embryological studies, and large scale EST sequences begin to emerge. We cloned five cytoplasmic intermediate filament (IF) cDNAs and made specific antibodies to the recombinant proteins. Self-assembly studies and immunofluorescence microscopy were used to study these proteins and their distribution. Confirming and extending previous studies in Styela, we found that Ciona protein IF-A is expressed in muscle and forms homopolymeric filaments while proteins IF-C and IF-D, which form only obligatory heteropolymeric filaments, resemble a keratin pair exclusively found in the entire epidermis. Protein IF-B and the new protein IF-F potentially reflect tunicate-specific IF proteins. They are found in the entire internal epithelia including the neural gland. We also extended the analysis to earlier developmental stages of Ciona. Protein IF-A is expressed in muscle from larval stages, whereas proteins IF-C and IF-D are found only in the tail epidermis. Protein IF-F is detected abundantly in the test cells of eggs, embryos and premetamorphic larvae. Our studies show that IF proteins could prove very useful markers in the study of cell fate determination in Ciona. They also support previous findings on the evolutionary relationships of different IF proteins. Non-vertebrate chordates have IF proteins which represent orthologs of vertebrate type I to III proteins, but also IF proteins that do not seem to fit into these classes. However, the intron positions of all tunicate IF genes are conserved with vertebrate type I to III genes, pointing to a common evolutionary origin.
机译：urochordate Ciona intestinalis是一个完善的胚胎学研究系统，并且大规模的EST序列开始出现。我们克隆了五个细胞质中间丝（IF）cDNA，并制备了针对重组蛋白的特异性抗体。自组装研究和免疫荧光显微镜研究了这些蛋白质及其分布。证实并扩展了以前在Styela的研究，我们发现Ciona蛋白IF-A在肌肉中表达并形成均聚物细丝，而仅形成强制性杂聚物细丝的IF-C和IF-D蛋白类似于仅在整个过程中发现的角蛋白对。表皮。蛋白质IF-B和新的蛋白质IF-F可能反映了被膜特异性的IF蛋白。它们在包括神经腺在内的整个内部上皮细胞中发现。我们还将分析扩展到了Ciona的早期开发阶段。 IF-A蛋白在幼虫期的肌肉中表达，而IF-C和IF-D蛋白仅在尾部表皮中发现。在鸡蛋，胚胎和亚变态幼虫的测试细胞中大量检测到了IF-F蛋白。我们的研究表明，IF蛋白可以证明在Ciona细胞命运确定研究中非常有用的标记。他们还支持先前关于不同IF蛋白进化关系的发现。非脊椎动物脊索动物具有代表脊椎动物I至III型蛋白质直系同源物的IF蛋白，但也有似乎不适合这些类别的IF蛋白。但是，所有被膜IF基因的内含子位置与脊椎动物I至III型基因保守，这表明其共同的进化起源。

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