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首页> 外文期刊>Immunology Letters >Allergen-specific regulation of allergic rhinitis in mice by intranasal exposure to IgG1 monoclonal antibody Fab fragments against pathogenic allergen
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Allergen-specific regulation of allergic rhinitis in mice by intranasal exposure to IgG1 monoclonal antibody Fab fragments against pathogenic allergen

机译：鼻内暴露于针对致病性变应原的IgG1单克隆抗体Fab片段，可对小鼠变应性鼻炎进行变应原特异性调节

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摘要

Fab fragments (Fabs) have the ability to bind to specific antigens but lack the Fc portion for binding to receptors on immune and inflammatory cells that play a critical role in allergic diseases. In the present study, we investigated whether Fabs of an allergen-specific IgG1 monoclonal antibody (mAb) inhibited allergic rhinitis in mice. BALB/c mice sensitized by intraperitoneal injections of ovalbumin (OVA) plus alum on days 0 and 14 were intranasally challenged with OVA on days 28-30, and 35. Fabs prepared by the digestion of an anti-OVA IgG1 mAb (O1-10) with papain were also intranasally administered 15. min before each OVA challenge. The results showed that treatment with O1-10 Fabs significantly suppressed the sneezing frequency, associated with decrease of OVA-specific IgE in the serum and infiltration by mast cells in the nasal mucosa seen following the fourth antigenic challenge additionally, the level of mouse mast cell protease-1, a marker of mast cell activation, in serum was decreased. Furthermore, infiltration of eosinophils and goblet cell hyperplasia in the nasal mucosa at the fourth challenge were inhibited by treatment with O1-10 Fabs. In conclusion, these results suggest that intranasal exposure to Fabs of a pathogenic antigen-specific IgG1 mAb may be effective in regulating allergic rhinitis through allergen capture by Fabs in the nasal mucosa before the interaction of the intact antibody and allergen.
机译：Fab片段（Fabs）具有与特定抗原结合的能力，但缺少与免疫和炎性细胞上的受体结合的Fc部分，这些细胞在变应性疾病中起关键作用。在本研究中，我们调查了过敏原特异性IgG1单克隆抗体（mAb）的Fabs是否能抑制小鼠的过敏性鼻炎。在第0天和第14天通过腹膜内注射卵清蛋白（OVA）加明矾致敏的BALB / c小鼠在第28-30天和第35天用OVA鼻内攻击。通过消化抗OVA IgG1 mAb（O1-10 ）在每次OVA攻击前15分钟也要鼻内给予木瓜蛋白酶。结果表明，用O1-10 Fabs处理可显着抑制打喷嚏频率，与血清中OVA特异性IgE的降低和第四次抗原挑战（即小鼠肥大细胞的水平）后鼻黏膜肥大细胞浸润有关血清中肥大细胞激活的标志物蛋白酶1降低。此外，第四次攻击时鼻黏膜中嗜酸性粒细胞的浸润和杯状细胞增生被O1-10 Fabs抑制。总之，这些结果表明，在完整抗体与变应原相互作用之前，鼻内暴露于病原性抗原特异性IgG1 mAb的Fab可能通过Fab在鼻粘膜中捕获变应原而有效地调节变应性鼻炎。

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