...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Immunology: An Official Journal of the British Society for Immunology >Differentiation and activation of equine monocyte-derived dendritic cells are not correlated with CD206 or CD83 expression
【24h】

Differentiation and activation of equine monocyte-derived dendritic cells are not correlated with CD206 or CD83 expression

机译：马单核细胞衍生的树突状细胞的分化和激活与CD206或CD83表达无关

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Dendritic cells (DC) are the main immune mediators inducing primary immune responses. DC generated from monocytes (MoDC) are a model system to study the biology of DC in vitro, as they represent inflammatory DC in vivo. Previous studies on the generation of MoDC in horses indicated that there was no distinct difference between immature and mature DC and that the expression profile was distinctly different from humans, where CD206 is expressed on immature MoDC whereas CD83 is expressed on mature MoDC. Here we describe the kinetics of equine MoDC differentiation and activation, analysing both phenotypic and functional characteristics. Blood monocytes were first differentiated with equine granulocyte-macrophage colony-stimulating factor and interleukin-4 generating immature DC (iMoDC). These cells were further activated with a cocktail of cytokines including interferon-γ) but not CD40 ligand to obtain mature DC (mMoDC). To determine the expression of a broad range of markers for which no monoclonal antibodies were available to analyse the protein expression, microarray and quantitative PCR analysis were performed to carry out gene expression analysis. This study demonstrates that equine iMoDC and mMoDC can be distinguished both phenotypically and functionally but the expression pattern of some markers including CD206 and CD83 is dissimilar to the human system.
机译：树突状细胞（DC）是诱导主要免疫反应的主要免疫介质。单核细胞产生的DC（MoDC）是一个模型系统，用于研究DC的体外生物学，因为它们代表体内炎症性DC。先前关于马中MoDC产生的研究表明，未成熟和成熟DC之间没有明显差异，并且表达谱与人明显不同，其中CD206在未成熟MoDC上表达，而CD83在成熟MoDC上表达。在这里，我们描述了马MoDC分化和激活的动力学，分析了表型和功能特征。首先用马粒细胞-巨噬细胞集落刺激因子和白细胞介素4生成未成熟DC（iMoDC）分化血液单核细胞。这些细胞进一步用包括干扰素-γ（但不包括CD40配体）的细胞因子混合物激活，以获得成熟的DC（mMoDC）。为了确定没有单克隆抗体可用于分析蛋白质表达的多种标记物的表达，进行了微阵列和定量PCR分析以进行基因表达分析。这项研究表明，马iMoDC和mMoDC可以在表型和功能上加以区分，但是某些标记（包括CD206和CD83）的表达模式与人类系统不同。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号