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首页> 外文期刊>Immunology: An Official Journal of the British Society for Immunology >Differential chemokine and cytokine production by neonatal bovine γδ T-cell subsets in response to viral toll-like receptor agonists and in vivo respiratory syncytial virus infection
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Differential chemokine and cytokine production by neonatal bovine γδ T-cell subsets in response to viral toll-like receptor agonists and in vivo respiratory syncytial virus infection

机译：新生牛γδT细胞亚群对病毒toll样受体激动剂和体内呼吸道合胞病毒感染的反应性趋化因子和细胞因子产生差异

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γδ T cells respond to stimulation via toll-like receptors (TLR). Bovine γδ T cells express TLR3 and TLR7, receptors that are key for the recognition of viruses such as bovine respiratory syncytial virus (BRSV); however, responses of γδ T cells to stimulation via these receptors, and their role during viral infections, remains unclear. Here, we demonstrate that neonatal bovine γδ T cells exhibit robust chemokine and cytokine production in response to the TLR3 agonist, Poly(I:C), and the TLR7 agonist, Imiquimod. Importantly, we observe a similar phenotype in γδ T-cell subsets purified from calves infected with BRSV. Bovine γδ T cells are divided into subsets based upon their expression of WC1, and the response to TLR stimulation and viral infection differs between these subsets, with WC1.1+ and WC1neg γδ T cells producing macrophage inflammatory protein-1α and granulocyte-macrophage colony-stimulating factor, and WC1.2+ γδ T cells preferentially producing the regulatory cytokines interleukin-10 and transforming growth factor-β. We further report that the active vitamin D metabolite 1,25-dihydroxyvitamin D3 does not alter γδ T-cell responses to TLR agonists or BRSV. To our knowledge, this is the first characterization of the γδ T-cell response during in vivo BRSV infection and the first suggestion that WC1.1+ and WC1neg γδ T cells contribute to the recruitment of inflammatory populations during viral infection. Based on our results, we propose that circulating γδ T cells are poised to rapidly respond to viral infection and suggest an important role for γδ T cells in the innate immune response of the bovine neonate.
机译：γδT细胞通过收费样受体（TLR）响应刺激。牛γδT细胞表达TLR3和TLR7受体，这是识别病毒（如牛呼吸道合胞病毒（BRSV））的关键。然而，尚不清楚γδT细胞通过这些受体对刺激的反应及其在病毒感染中的作用。在这里，我们证明了新生儿牛γδT细胞表现出强大的趋化因子和细胞因子产生，以响应TLR3激动剂Poly（I：C）和TLR7激动剂咪喹莫特。重要的是，我们在从感染了BRSV的牛犊中纯化的γδT细胞亚群中观察到相似的表型。牛γδT细胞根据其WC1的表达分为亚类，并且这些子集之间对TLR刺激和病毒感染的反应不同，其中WC1.1 +和WC1negγδT细胞产生巨噬细胞炎性蛋白-1α和粒细胞-巨噬细胞集落刺激因子和WC1.2 +γδT细胞优先产生调节性细胞因子白介素10和转化生长因子β。我们进一步报告，活性维生素D代谢物1,25-二羟基维生素D3不会改变TLR激动剂或BRSV的γδT细胞反应。据我们所知，这是体内BRSV感染期间γδT细胞反应的第一个特征，也是WC1.1 +和WC1negγδT细胞在病毒感染过程中促进炎症种群募集的第一个提示。根据我们的研究结果，我们认为循环中的γδT细胞可以对病毒感染做出快速反应，并建议γδT细胞在牛新生儿的先天免疫反应中起重要作用。

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