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首页> 外文期刊>Immunology: An Official Journal of the British Society for Immunology >A subset of functional effector-memory CD8+ T lymphocytes in human immunodeficiency virus-infected patients.
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A subset of functional effector-memory CD8+ T lymphocytes in human immunodeficiency virus-infected patients.

机译：人类免疫缺陷病毒感染患者的功能性效应记忆性CD8 + T淋巴细胞的子集。

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CD8(+) T cells provide protective immune responses via both cytolytic and non-cytolytic mechanisms in subjects infected with human immunodeficiency virus (HIV). In the present study, we investigated the CD28 expression of CD8(+) T cells present in the peripheral blood lymphocyte subset isolated from chronically HIV-infected subjects. Using flow cytometric analysis, a continuous spectrum of CD28 intensity ranging from negative to high, which could be separated into CD28-negative, intermediate (int) and high, was seen for CD8(+) T cells. Our study focused mostly on the CD28(int) CD8(+) T cells. The CD28(int) CD8(+) T cells are CD57(-) CD27(+) CD45RO(+) CD45RA(-) CCR7(low) CD62L(int). The proliferative capacity of CD28(int) CD8(+) T cells was intermediate between those of CD28(-) CD8(+) T cells and CD28(high) CD8(+) T cells. The CD28(int) CD8(+) T cells are specific for HIV, cytomegalovirus (CMV) and Epstein-Barr virus (EBV) antigens as measured by human leucocyte antigen pentamer binding and produce bothintracellular interferon-gamma and tumour necrosis factor-alpha in response to their cognate viral peptides. The CD28(int) CD8(+) T cells have HIV-specific, CMV-specific and EBV-specific cytotoxic activity in response to their cognate viral peptides. These findings indicate that a subset of functional effector-memory CD8(+) T cells specific for HIV, CMV and EBV antigens may contribute to an efficient immune response in HIV-infected subjects.
机译：CD8（+）T细胞通过溶细胞和非溶细胞机制在感染人类免疫缺陷病毒（HIV）的受试者中提供保护性免疫应答。在本研究中，我们调查了从慢性HIV感染者分离的外周血淋巴细胞亚群中存在的CD8（+）T细胞的CD28表达。使用流式细胞仪分析，可以看到CD28强度从负到高的连续谱图，对于CD8（+）T细胞，可以看到它可以分为CD28负，中间（int）和高。我们的研究主要集中在CD28（int）CD8（+）T细胞上。 CD28（int）CD8（+）T细胞是CD57（-）CD27（+）CD45RO（+）CD45RA（-）CCR7（低）CD62L（int）。 CD28（int）CD8（+）T细胞的增殖能力介于CD28（-）CD8（+）T细胞和CD28（high）CD8（+）T细胞之间。 CD28（int）CD8（+）T细胞对HIV，巨细胞病毒（CMV）和爱泼斯坦巴尔病毒（EBV）抗原具有特异性，通过人白细胞抗原五聚体结合测量，并在细胞内产生细胞内干扰素-γ和肿瘤坏死因子-α对它们的同源病毒肽的应答。 CD28（int）CD8（+）T细胞响应其同源病毒肽，具有HIV特异性，CMV特异性和EBV特异性的细胞毒活性。这些发现表明，特异于HIV，CMV和EBV抗原的功能性效应记忆CD8（+）T细胞的子集可能有助于HIV感染者的有效免疫应答。

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