Identification of MHC class II restricted T-cell-mediated reactivity against MHC class I binding Mycobacterium tuberculosis peptides.

Wang M; Tang ST; Stryhn A; Justesen S; Larsen MV; Dziegiel MH; Lewinsohn DM; Buus S; Lund O; Claesson MH

首页> 外文期刊>Immunology: An Official Journal of the British Society for Immunology >Identification of MHC class II restricted T-cell-mediated reactivity against MHC class I binding Mycobacterium tuberculosis peptides.

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Identification of MHC class II restricted T-cell-mediated reactivity against MHC class I binding Mycobacterium tuberculosis peptides.

机译：鉴定MHC II类对结合MHC I类结核分枝杆菌肽的T细胞介导的反应性。

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Major histocompatibility complex (MHC) class I restricted cytotoxic T lymphocytes (CTL) are known to play an important role in the control of Mycobacterium tuberculosis infection so identification of CTL epitopes from M. tuberculosis is of importance for the development of effective peptide-based vaccines. In the present work, bioinformatics technology was employed to predict binding motifs of 9mer peptides derived from M. tuberculosis for the 12 HLA-I supertypes. Subsequently, the predicted peptides were synthesized and assayed for binding to HLA-I molecules in a biochemically based system. The antigenicity of a total of 157 peptides with measured affinity for HLA-I molecules of K(D)

机译：已知主要的组织相容性复合物（MHC）I类限制性细胞毒性T淋巴细胞（CTL）在结核分枝杆菌感染的控制中起着重要作用，因此从结核分枝杆菌中鉴定CTL表位对于开发有效的基于肽的疫苗非常重要。在当前的工作中，采用生物信息学技术来预测源自结核分枝杆菌的9mer肽对12种HLA-1超型的结合基序。随后，合成了预测的肽，并在基于生物化学的系统中测定了与HLA-1分子的结合。使用来自高度纯化的蛋白衍生物反应性健康供体的外周血T细胞，评估了对157个对K（D）≤= 500 nM的HLA-1分子具有亲和力的肽的抗原性。在157个肽中，发现有8个肽（5％）可诱导T细胞反应。从在ELISPOT分析培养中用HLA I和II类亚型抗体阻断来判断，这八种抗原肽均未诱导HLA I类限制CD8（+）T细胞应答。相反，所有反应均被泛HLA II类和抗HLA-DR抗体阻断。此外，在扩增10天之前，CD4（+）T细胞耗竭，导致ELISPOT培养物中大多数肽特异性的反应性完全丧失。在结核分枝杆菌肽存在下扩大的T细胞的细胞内干扰素-γ染色的FACS分析证实，应答细胞的确是CD4（+）。总之，在许多情况下，针对HLA-I结合9mer结核分枝杆菌衍生肽的T细胞免疫可能被CD4（+）T细胞介导，并受到HLA-II分子的限制。 CD8（+）和CD4（+）T细胞都识别的9mer肽的使用对于未来基于结核分枝杆菌肽的疫苗的开发可能很重要。

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《Immunology: An Official Journal of the British Society for Immunology》 |2011年第4期|共10页
作者
Wang M; Tang ST; Stryhn A; Justesen S; Larsen MV; Dziegiel MH; Lewinsohn DM; Buus S; Lund O; Claesson MH;
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正文语种 eng
中图分类医学免疫学;
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1. Identification of MHC class II restricted T-cell-mediated reactivity against MHC class I binding Mycobacterium tuberculosis peptides. [J] . Wang M, Tang ST, Stryhn A, Immunology: An Official Journal of the British Society for Immunology . 2011,第4期

机译：鉴定MHC II类对结合MHC I类结核分枝杆菌肽的T细胞介导的反应性。
2. Thymus dictates major histocompatibility complex (MHC) specificity and immune response gene phenotype of class II MHC-restricted T cells but not of class I MHC-restricted T cells. [J] . W M Kast, L P de Waal, C J Melief The Journal of Experomental Medicine . 1984,第6期

机译：胸腺决定了II类MHC限制性T细胞的主要组织相容性复合物（MHC）特异性和免疫应答基因表型，但不决定I类MHC限制性T细胞的免疫应答基因表型。
3. Predicting promiscuous antigenic T cell epitopes of Mycobacterium tuberculosis mymA operon proteins binding to MHC Class I and Class II molecules [J] . Saraav Iti, Pandey Kirti, Sharma Monika, Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases . 2016,第Null期

机译：预测结核分枝杆菌mymA操纵子蛋白与MHC I类和II类分子结合的混杂T细胞抗原表位
4. Hyperactivation of gp130-mediated STAT3 signaling induces a rheumatoid arthritis-like disease that is dependent on MHC class II restricted CD4+ T cells [C] . Masaaki Murakami, Shin-ichiro Sawa, Daisuke Kamimura, Uehara Memorial Foundation Symposium on the Innate Immune System . 2005

机译：GP130介导的STAT3信号传导的多动激活诱导类风湿性关节炎样疾病，其依赖于MHC II类限制的CD4 + T细胞
5. Immune evasion by Mycobacterium tuberculosis: Mycobacterial lipoproteins exploit Toll-like receptor-2 signaling to inhibit class-II MHC antigen processing. [D] . Gehring, Adam J. 2004

机译：结核分枝杆菌的免疫逃逸：分枝杆菌脂蛋白利用Toll样受体2信号转导抑制II类MHC抗原的加工。
6. Identification of MHC class II restricted T-cell-mediated reactivity against MHC class I binding Mycobacterium tuberculosis peptides [O] . Mingjun Wang, Sheila T Tang, Anette Stryhn, 2011

机译：鉴定MHC II类受限制的T细胞介导的针对MHC I类结合结核分枝杆菌肽的反应性
7. Identification of MHC class II restricted T-cell-mediated reactivity against MHC class I binding Mycobacterium tuberculosis peptides [O] . Wang, Mingjun, Tang, Sheila T, Stryhn, Anette, 2011

机译：鉴定MHC II类受限制的T细胞介导的针对MHC I类结合结核分枝杆菌肽的反应性
8. Activation of MyD88 Signaling upon Staphylococcal Enterotoxin Binding to MHC Class II Molecules [R] . Kissner, T. L., Ruthel, G., Alam, S., 2011

机译：金黄色葡萄球菌肠毒素与mHC II类分子结合后myD88信号的激活

Identification of MHC class II restricted T-cell-mediated reactivity against MHC class I binding Mycobacterium tuberculosis peptides.

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