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首页> 外文期刊>Immunology: An Official Journal of the British Society for Immunology >Distribution and development of the postnatal murine Vdelta1 T-cell receptor repertoire
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Distribution and development of the postnatal murine Vdelta1 T-cell receptor repertoire

机译：产后鼠Vdelta1 T细胞受体库的分布与发育

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摘要

Murine gamma/dekta T cells express canonical Vgamma5Vdeltal chains in the epidermis and Vgamma6Vdeltal chains at reproductive sites. Both subsets carry an identical Vdelta1-Ddelta2-Jdelta2 chain which completely lacks junctional diversity. These cells are thought to monitor tissue integrity via recognition of stress-induced self antigens. In this study, we showed by reverse transcription-polymerase chain reaction (RT-PCR), complementarity determining region 3 (CDR3) spectratyping and sequencing of the junctional regions of Vdelta1 chains from C57BL/6 mice (aged 1 day to 14 months) that the canonical Vdelta1-Ddelta2-Jdelta2 chain is also consistently present at other sites such as the thy-mus, gut, lung, liver, spleen and peripheral blood. In addition, we found multiple Vdelta1 chains with fetal type rearrangements which were also shared among organs and among animals. These Vdelta1 chains were typically characterized by a conserved amino acid motif, 'GGIRA'. Furthermore, by analysing the early postnatal period at days 10 and 16, we demonstrated that the diversification of the thymic Vdelta1 repertoire is not paralleled by a diversification of extrathymic Vdelta1+gamma/delta T cells. This indicates that only fetal type rearrangements survive at extrathymic sites. In conclusion, gamma/delta T cells expressing the canonical Vdeltal-Ddelta2-Jdelta2 chain are not unique to the skin and reproductive sites. Furthermore, we found other gamma/delta T cells expressing fetal type Vdelta1 chains which were shared among different organs and animals. Thus, gamma/delta T cells expressing conserved Vdelta1 chains are likely to have important functions. We suggest a model in which this subset continuously recirculates throughout the organism and rapidly responds to stress-induced self antigens.
机译：鼠γ/ dekta T细胞在表皮表达典型的Vgamma5Vdeltal链，在生殖部位表达Vgamma6Vdeltal链。两个子集都带有完全相同的Vdelta1-Ddelta2-Jdelta2链，该链完全缺乏连接多样性。这些细胞被认为通过识别应激诱导的自身抗原来监测组织完整性。在这项研究中，我们通过逆转录聚合酶链反应（RT-PCR），互补决定区3（CDR3）谱型分析和C57BL / 6小鼠（1天至14个月）Vdelta1链连接区的测序表明，典范的Vdelta1-Ddelta2-Jdelta2链也始终存在于其他部位，例如胸腺，肠道，肺，肝，脾和外周血。此外，我们发现了多个具有胎儿类型重排的Vdelta1链，这些链在器官和动物之间也共享。这些Vdelta1链通常以保守的氨基酸基序'GGIRA'为特征。此外，通过分析出生后第10天和第16天的时间，我们证明胸腺Vdelta1血库的多样化与胸腺Vdelta1 +γ/δT细胞的多样化并没有平行。这表明只有胎儿类型的重排在胸腺外部位存活。总之，表达经典的Vdeltal-Ddelta2-Jdelta2链的γ/δT细胞并不是皮肤和生殖部位特有的。此外，我们发现其他表达胎儿型Vdelta1链的γ/δT细胞在不同的器官和动物之间共享。因此，表达保守的Vdelta1链的γ/δT细胞可能具有重要的功能。我们建议建立一个模型，其中该子集在整个生物体内不断循环并快速响应压力诱导的自身抗原。

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