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首页> 外文期刊>Immunology: An Official Journal of the British Society for Immunology >The CD27(+) memory B cells display changes in the gene expression pattern in elderly individuals
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The CD27(+) memory B cells display changes in the gene expression pattern in elderly individuals

机译：CD27（+）记忆B细胞显示老年人基因表达模式的变化

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摘要

Memory B cells (MBCs) have a long lifespan compared with naive B cells (NBCs), remaining viable for years. This could predispose them to suffer misbalances in the gene expression pattern in the long term, which might be involved in the development of age-related B-cell disorders. In order to identify genes whose expression might change during life, we analysed the gene expression patterns of CD27(-) NBCs versus CD27(+) MBCs in young and old subjects. Using microarray assays we observed that the expression pattern of CD27(-) NBCs versus CD27(+) MBCs is significantly different. Furthermore, to evaluate the age effect, we compared the gene expression pattern of young versus aged subjects in both cell populations. Interestingly, we did not find significant differences in the CD27(-) NBC population between young and aged individuals, whereas we found 925 genes differentially expressed in CD27(+) MBCs. Among these genes, 193 were also differentially expressed in CD27(+) MBCs compared with CD27(-) NBCs, most of them involved in cell survival, cell growth and proliferation, cellular development and gene expression. We conclude that gene expression profiles of CD27(-) NBCs and CD27(+) MBCs are different. Moreover, whereas the gene expression pattern of CD27(+) MBCs varies with age, the same does not happen in CD27(-) NBCs. This suggests that MBCs undergo time-dependent changes, which could underlie a higher susceptibility to dysfunction with age.
机译：与幼稚B细胞（NBC）相比，记忆B细胞（MBC）的寿命长，并且可以存活数年。从长远来看，这可能使他们容易遭受基因表达模式的失衡，这可能与年龄相关的B细胞疾病的发展有关。为了鉴定其表达可能在生活中发生变化的基因，我们分析了CD27（-）NBC与CD27（+）MBC在年轻人和老年人中的基因表达模式。使用微阵列分析，我们观察到CD27（-）NBC与CD27（+）MBC的表达模式显着不同。此外，为了评估年龄效应，我们比较了两个细胞群中年轻受试者和老年受试者的基因表达模式。有趣的是，我们没有发现年轻人和老年人之间CD27（-）NBC群体的显着差异，而我们发现了在CD27（+）MBC中差异表达的925个基因。在这些基因中，与CD27（-）NBC相比，在CD27（+）MBC中也有193个差异表达，其中大多数与细胞存活，细胞生长和增殖，细胞发育和基因表达有关。我们得出结论，CD27（-）NBC和CD27（+）MBC的基因表达谱是不同的。此外，尽管CD27（+）MBC的基因表达模式随年龄而变化，但CD27（-）NBC却没有。这表明MBC经历了时间依赖性的变化，这可能是随着年龄增长而对功能障碍的更高敏感性。

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