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首页> 外文期刊>European Journal of Plant Pathology >Generation of polyclonal antibodies and serological analyses of nucleocapsid protein of Soybean vein necrosis-associated virus: A distinct soybean infecting tospovirus serotype
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Generation of polyclonal antibodies and serological analyses of nucleocapsid protein of Soybean vein necrosis-associated virus: A distinct soybean infecting tospovirus serotype

机译：大豆静脉坏死相关病毒的多克隆抗体的产生和核衣壳蛋白的血清学分析：一种感染弓形病毒血清型的独特大豆

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摘要

Soybean vein necrosis-associated virus (SVNaV) is a newly isolated tospovirus from field-grown soybeans in the United States. Polyclonal antibodies generated against the recombinant Escherichia coli-expressed nucleocapsid protein (NP) of the virus, reacted specifically with SVNaV and exhibited low, if any, cross-reactivity with other species within the genus Tospovirus. The serological results are in agreement with low sequence homology of the SVNaV-NP gene when compared with those of other tospovirus species, some of which are capable of infecting soybean naturally. Phylogenetic analysis utilizing NP sequences from several SVNaV isolates collected from different geographical regions and various soybean genotypes over 4 years showed close relationships, but distinct from the representatives of all the established serogroups or distinct serotypes within the genus of Tospovirus. All SVNaV isolates examined reacted strongly with the generated polyclonal antibodies. Collectively, our serological analyses suggest that SVNaV represents a new and distinct soybean-infecting tospovirus serotype. Furthermore, our data demonstrate that antiserum against NP has the potential to serve as a reliable probe for SVNaV detection in field-grown soybeans.
机译：大豆静脉坏死相关病毒（SVNaV）是从美国田间种植的大豆中分离出来的一种新的弓形病毒。针对该病毒的重组大肠杆菌表达的核衣壳蛋白（NP）生成的多克隆抗体与SVNaV特异性反应，并且与Tospovirus属中的其他物种表现出低的交叉反应性（如果有的话）。与其他弓形病毒种类相比，血清学结果与SVNaV-NP基因的低序列同源性是一致的，其中一些能够自然感染大豆。利用从不同地理区域和各种大豆基因型收集的数种SVNaV分离株的NP序列进行的系统发育分析，在4年中显示出密切的关系，但不同于所有的已建立的血清群的代表或Tospovirus属内不同血清型的代表。检查的所有SVNaV分离株均与生成的多克隆抗体强烈反应。总的来说，我们的血清学分析表明，SVNaV代表了一种新的和独特的感染大豆的脊灰病毒血清型。此外，我们的数据表明，针对NP的抗血清具有作为田间种植的大豆SVNaV检测的可靠探针的潜力。

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