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首页> 外文期刊>European Journal of Plant Pathology >Characterization and pathogenicity of Pestalotiopsis uvicola causing black leaf spot on carob (Ceratonia siliqua L.) in Italy
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Characterization and pathogenicity of Pestalotiopsis uvicola causing black leaf spot on carob (Ceratonia siliqua L.) in Italy

机译：在意大利形成角豆（Ceratonia siliqua L.）的黑斑病的Pestalotiopsis uvicola的特征和致病性

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摘要

During spring 2013, several carob plants (Ceratonia siliqua L.), showing symptoms of necrotic black lesions on leaves, were observed in Capri Island, Southern Italy. Acervuli, producing conidia with septa and appendages, appeared in the centre of the lesions. In this report, the identification of the causal agent of this disease was made applying Koch's postulates. Morphological characteristics of colony (colour and mycelium appearance) and conidia (size, shape, number of septa, length and number of apical and basal appendages) were characterized to identify the pathogen. Moreover, for a rapid and unambiguous identification of the fungal species, the internal transcribed spacers, comprising the 5.8 rDNA gene (ITS1-5.8-ITS2), were amplified by PCR, using the genomic DNA extracted from the isolated colonies, and sequenced. The fungus isolated was ascribed to the species Pestalotiopsis uvicola (Speg.) Bissett. Phylogenetic analysis, performed with sequences present in GenBank database, showed that carob isolates clustered with P. uvicola isolates from North America and Italy, separately from Australian, Indian and Chinese isolates. P. uvicola isolates were pathogenic when artificially inoculated on carob leaves. To the best of our knowledge, this is the first report of P. uvicola on carob.
机译：2013年春季，在意大利南部的卡普里岛观察到数种角豆植物（Ceratonia siliqua L.），叶片上出现坏死性黑色病变的症状。在病变的中央出现了具间隔和附属物的分生孢子的子宫颈。在此报告中，根据科赫的假设确定了这种疾病的病因。菌落的形态学特征（颜色和菌丝体外观）和分生孢子（大小，形状，隔数，长度和根尖和基部附属物的数量）被鉴定为病原体。此外，为了快速明确地鉴定真菌，使用从分离的菌落中提取的基因组DNA，通过PCR扩增了包含5.8 rDNA基因（ITS1-5.8-ITS2）的内部转录间隔子。分离出的真菌归因于白僵菌（Pestalotiopsis uvicola（Speg。）Bissett）。用GenBank数据库中存在的序列进行的系统发育分析表明，角豆树分离株与来自北美和意大利的褐葡萄假单胞菌分离株成簇，与澳大利亚，印度和中国分离株分开。当人工接种在角豆树叶子上时，葡萄球菌分离株具有致病性。据我们所知，这是P. uvicola对角豆树的首次报道。

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