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首页> 外文期刊>European Journal of Plant Pathology >Botrytis cinerea induces senescence and is inhibited by autoregulated expression of the IPT gene.
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Botrytis cinerea induces senescence and is inhibited by autoregulated expression of the IPT gene.

机译：灰葡萄孢（Botrytis cinerea）诱导衰老并且被IPT基因的自动调节表达抑制。

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Botrytis cinerea is a non-specific, necrotrophic pathogen that attacks many plant species, including Arabidopsis and tomato. Since senescing leaves are particularly susceptible to infection by B. cinerea, we hypothesized that the fungus might induce senescence as part of its mode of action and that delaying leaf senescence might reduce the severity of B. cinerea infections. To examine these hypotheses, we followed the expression of Arabidopsis SAG12, a senescence-specific gene, upon infection with B. cinerea. Expression of SAG12 is induced by B. cinerea infection, indicating that B. cinerea induces senescence. The promoter of SAG12, as well as that of a second Arabidopsis senescence-associated gene, SAG13, whose expression is not specific to senescence, were previously analyzed in tomato plants and were found to be expressed in a similar manner in the two species. These promoters were previously used in tomato plants to drive the expression of isopentenyl transferase (IPT) from Agrobacterium to suppress leaf senescence (Swartzberg et al. in Plant Biology 8:579-586, 2006). In this study, we examined the expression of these promoters following infection of tomato plants with B. cinerea. Both promoters exhibit high expression levels upon B. cinerea infection of non-senescing leaves of tomato plants, supporting our conclusion that B. cinerea induces senescence as part of its mode of action. In contrast to B. cinerea, Trichoderma harzianum T39, a saprophytic fungus that is used as a biocontrol agent against B. cinerea, induces expression of SAG13 only. Expression of IPT, under the control of the SAG12 and SAG13 promoters in response to infection with B. cinerea resulted in suppression of B. cinerea-induced disease symptoms, substantiating our prediction that.
机译：灰葡萄孢是一种非特异性的坏死性病原体，攻击许多植物，包括拟南芥和番茄。由于衰老的叶片特别容易受到灰质双歧杆菌的感染，因此我们假设真菌可能会诱导衰老，这是其作用方式的一部分，而延迟叶片的衰老可能会降低灰质双歧杆菌的感染严重性。为了检查这些假设，我们跟踪了灰质芽孢杆菌感染后拟南芥SAG12（一种衰老特异性基因）的表达。灰葡萄孢杆菌感染诱导了SAG12的表达，表明灰葡萄芽孢杆菌诱导衰老。 SAG12的启动子，以及第二个拟南芥衰老相关基因SAG13的启动子，其表达对衰老不是特异性的，先前已经在番茄植株中进行了分析，发现在两个物种中以相似的方式表达。这些启动子先前在番茄植物中用于驱动来自农杆菌的异戊烯基转移酶（IPT）的表达以抑制叶片衰老（Swartzberg等人，Plant Biology 8：579-586，2006）。在这项研究中，我们检查了番茄芽孢杆菌感染番茄植物后这些启动子的表达。这两个启动子在番茄红素感染番茄的非衰老叶片后均表现出高表达水平，支持了我们的结论，即番茄红素诱导衰老是其作用方式的一部分。与灰质芽孢杆菌相反，哈茨木霉T39（一种腐生真菌，被用作针对灰霉芽孢杆菌的生物防治剂）仅诱导SAG13的表达。 IPT在SAG12和SAG13启动子的控制下，响应灰葡萄孢菌感染而表达，可抑制灰葡萄孢菌引起的疾病症状，从而证实了我们的预测。

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