...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>European Journal of Plant Pathology >The development of a PCR-based method for detecting Puccinia striiformis latent infections in wheat leaves.
【24h】

The development of a PCR-based method for detecting Puccinia striiformis latent infections in wheat leaves.

机译：基于PCR的小麦叶片条锈病潜在感染检测方法的开发。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Stripe rust of wheat caused by Puccinia striiformis f. sp. tritici is one of the most important diseases on wheat worldwide, especially in temperate regions with cool moist weather conditions. A rapid and reliable detection of the pathogen in latent infected wheat leaves during overwintering of the fungus in the dormant stage will contribute to determine the initial inoculum potential and thus to predict early outbreak and to improve effective management of the disease. To achieve this aim, a PCR-based method was developed for specific and sensitive detection of P. striiformis. Specific primers were designed according to a genome-specific sequence of P. striiformis. To evaluate the specificity of the primers, seven different isolates and races of P. striiformis as well as six other pathogens of wheat were tested. All isolates of P. striiformis yielded a distinct band of a fragment of 470 bp, while using DNA of the other wheat pathogens as a template no amplification product was detected. The sensitivity of the primers was tested using serial dilutions of total DNA from P. striiformis; the limit of detection was 10 pg of DNA. Using extracts from P. striiformis-infected wheat leaves, the fungus could be determined in the leaves before symptoms appeared. The stripe rust could also be detected in the dormant stage by the PCR assay in samples of wheat leaves taken during the winter season. The application of the PCR assay may be useful for rapid and reliable detection of P. striiformis in latent infected leaves of overwintering wheat plants.
机译：小麦条锈菌引起的小麦条锈病f。 sp。小麦是全世界小麦最重要的病害之一，尤其是在天气凉爽潮湿的温带地区。在休眠期真菌越冬过程中，快速，可靠地检测潜伏感染的小麦叶片中的病原体将有助于确定最初的接种潜力，从而预测早期爆发并改善对该病的有效管理。为了实现这一目标，开发了一种基于PCR的方法来特异性和灵敏地检测条形假单胞菌。根据条状疟原虫的基因组特异性序列设计特异性引物。为了评估引物的特异性，测试了七种不同的条状假单胞菌以及小麦的六个病原体。所有分离的条锈菌产生一条明显的470 bp的条带，而使用其他小麦病原体的DNA作为模板，未检测到扩增产物。引物的敏感性使用条纹状毕赤酵母总DNA的连续稀释液进行测试;检测限为10 pg DNA。使用来自被棒状线虫感染的小麦叶片的提取物，可以在症状出现之前测定叶片中的真菌。在冬季，也可以通过PCR检测在小麦叶片样品中在休眠阶段检测条锈。 PCR分析的应用对于快速和可靠地检测越冬小麦植株的潜伏感染叶片中的条状线虫可能有用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号