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Detection of invasive freshwater fish species using environmental DNA survey

机译：使用环境DNA调查检测侵入性淡水鱼物种

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Invasive species are one of the most significant problem in freshwater ecosystems. Most common non-native freshwater species in Turkish freshwater fish fauna are Prussian Carp (Carassius gibelio), North African Catfish (Clarias gariepinus), Nile Tilapia (Oreochromis niloticus) and Topmouth Gudgeon (Pseudorasbora parva). Recent studies showed that environmental DNA could be used to detect target species inhabiting the ecosystem with higher precision and less effort compared to traditional field surveys. In this study, eDNA approach was used to investigate non-native freshwater fish species from fifteen different locations of Upper Sakarya Basin. eDNA was successfully extracted from the water samples of locations where the species were visually observed. Mean amplification rate of eDNA was calculated as 77.03%. This study is the first environmental DNA study used in detection of four of the most common invasive freshwater fish species. Results clearly indicating that eDNA surveys could be used as an important molecular tool to monitor invasive fish species in freshwater ecosystems
机译：入侵物种是淡水生态系统中最重要的问题之一。土耳其淡水鱼类动物群中最常见的非本地淡水物种是普鲁士鲤鱼（Carassius gibelio），北非Cat鱼（Clarias gariepinus），尼罗罗非鱼（Oreochromis niloticus）和Topmouth Gudgeon（Pseudorasbora parva）。最近的研究表明，与传统的田间调查相比，环境DNA可以用于以更高的精确度和更少的工作量检测生态系统中的目标物种。在这项研究中，使用eDNA方法研究了上萨卡里亚盆地15个不同地点的非本地淡水鱼类物种。从目测到的物种的水样中成功提取了eDNA。计算出eDNA的平均扩增率为77.03％。这项研究是用于检测四种最常见的入侵淡水鱼物种的第一项环境DNA研究。结果清楚表明，eDNA调查可作为监测淡水生态系统中入侵鱼类的重要分子工具

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