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首页> 外文期刊>Experimental and therapeutic medicine >Anti-proliferative effect of Flos Albiziae flavonoids on the human gastric cancer SGC-7901 cell line
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Anti-proliferative effect of Flos Albiziae flavonoids on the human gastric cancer SGC-7901 cell line

机译：黄花黄酮对人胃癌SGC-7901细胞的抗增殖作用

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摘要

The flavonoids found in many foods may have a protective effect against human gastric cancer. However, little information is available concerning the effects of flavonoids on the SGC-7901 cell line. Therefore, we first evaluated the effects of purified Flos Albiziae flavonoids (FAFs) on the proliferation of the SGC-7901 human gastric cancer cell line and investigated its possible anti-proliferative mechanisms. When SGC-7901 cells were treated with FAFs for various time periods (12-72 h) and at various doses (0-32 mu g/ml), cell growth decreased significantly in a time- and dose-dependent manner. Morphological observations with fluorescence microscopy and transmission electron microscopy (TEM) yielded clear evidence of cell shrinkage, formation of cytoplasmic filaments, condensation of nuclear chromatin, and cell apoptosis in the presence of FAFs. Treatment with FAFs changed the expression levels of Bcl-2, P65, Bax and caspase. The anti-apoptotic protein expression of Bcl-2 and p65 decreased gradually with the increase in FA F concentration, compared with control cells (P < 0.05). FAFs contributed to the increase in Bax and caspase expression. The expression of pro-apoptotic proteins Bax and caspase were upregulated by FAFs compared with control cells (P < 0.01). These results demonstrated that FAFs effectively induced apoptosis in the SGC-7901 cell line. This indicates that FAFs are likely to possess anticancer activity.
机译：在许多食物中发现的类黄酮可能对人类胃癌具有保护作用。但是，关于黄酮类化合物对SGC-7901细胞系的作用的信息很少。因此，我们首先评估了纯化的茜草黄酮类化合物（FAF）对SGC-7901人胃癌细胞系增殖的影响，并研究了其可能的抗增殖机制。当用FAF在不同时间段（12-72 h）和不同剂量（0-32μg / ml）对SGC-7901细胞进行处理时，细胞生长以时间和剂量依赖性方式显着下降。荧光显微镜和透射电子显微镜（TEM）的形态学观察得到了明显的证据，表明存在FAF时，细胞会收缩，细胞质细丝形成，核染色质凝结以及细胞凋亡。用FAFs处理可改变Bcl-2，P65，Bax和caspase的表达水平。与FA细胞相比，Bcl-2和p65的抗凋亡蛋白表达逐渐降低（P <0.05）。 FAF促进了Bax和caspase表达的增加。与对照组相比，FAFs可上调促凋亡蛋白Bax和caspase的表达（P <0.01）。这些结果证明FAF有效地诱导了SGC-7901细胞系的凋亡。这表明FAF可能具有抗癌活性。

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